eIF5A has a function in the elongation step of translation in yeast

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorGregio, Ana P. B.
dc.creatorCano, Veridiana P. S.
dc.creatorAvaca, Juliana S.
dc.creatorValentini, Sandro Roberto
dc.creatorZanelli, Cleslei Fernando
dc.date2014-05-20T13:24:15Z
dc.date2016-10-25T16:44:59Z
dc.date2014-05-20T13:24:15Z
dc.date2016-10-25T16:44:59Z
dc.date2009-03-20
dc.date.accessioned2017-04-05T19:59:33Z
dc.date.available2017-04-05T19:59:33Z
dc.identifierBiochemical and Biophysical Research Communications. San Diego: Academic Press Inc. Elsevier B.V., v. 380, n. 4, p. 785-790, 2009.
dc.identifier0006-291X
dc.identifierhttp://hdl.handle.net/11449/7475
dc.identifierhttp://acervodigital.unesp.br/handle/11449/7475
dc.identifier10.1016/j.bbrc.2009.01.148
dc.identifierWOS:000264271100013
dc.identifierhttp://dx.doi.org/10.1016/j.bbrc.2009.01.148
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/856108
dc.descriptionThe putative translation factor eIF5A is essential for cell viability and is highly conserved throughout evolution. Here, we describe genetic interactions between an eIF5A Mutant and a translation initiation mutant (eIF4E) or a translation elongation mutant (eEF2). Polysome profile analysis of single and double mutants revealed that mutation in eIF5A reduces polysome run-off, contrarily to translation initiation mutants. Moreover, the polysome profile of an eIF5A mutant alone is very similar to that of a translation elongation mutant. Furthermore, depletion of eIF5A causes a significant decrease in total protein synthesis and an increase of the average ribosome transit time. Finally, we, demonstrate that the formation of P bodies is inhibited in an eIF5A mutant, similarly to the effect of the translation elongation inhibitor cycloheximide. taken together, these results not only reinforce a role for eIF5A in translation but also strongly support a function for eIF5A in the elongation step of protein synthesis. (C) 2009 Elsevier B.V. All rights reserved.
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageeng
dc.publisherAcademic Press Inc. Elsevier B.V.
dc.relationBiochemical and Biophysical Research Communications
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjecteIF5A
dc.subjectTranslation elongation
dc.subjecteEF2
dc.subjectRibosome transit time
dc.subjectEF-P
dc.subjectHypusine
dc.titleeIF5A has a function in the elongation step of translation in yeast
dc.typeOtro


Este ítem pertenece a la siguiente institución