Immunostimulatory and cytotoxic activities of Indigofera suffruticosa (Fabaceae)

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorLopes, Flavia C. M.
dc.creatorCalvo, Tamara Regina
dc.creatorColombo, Lucas L.
dc.creatorVilegas, Wagner
dc.creatorCarlos, Iracilda Z.
dc.date2014-05-20T13:23:59Z
dc.date2014-05-20T13:23:59Z
dc.date2011-01-01
dc.date.accessioned2017-04-05T19:59:01Z
dc.date.available2017-04-05T19:59:01Z
dc.identifierNatural Product Research. Abingdon: Taylor & Francis Ltd, v. 25, n. 19, p. 1796-1806, 2011.
dc.identifier1478-6419
dc.identifierhttp://hdl.handle.net/11449/7347
dc.identifier10.1080/14786419.2010.488624
dc.identifierWOS:000299692600003
dc.identifier0000-0003-3032-2556
dc.identifierhttp://dx.doi.org/10.1080/14786419.2010.488624
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/856040
dc.descriptionOne of the major disadvantages of the current cancer therapy is the suppression of the immune system. Brazilian flora is considered one of the most diverse in the world and many plants were found to contain active constituents that can be valuable sources of new drugs. The plant Indigofera suffruticosa was studied to determine its potential to stimulate the immune system and also to be effective against tumour cells. We investigated the effects of the alkaloidal fraction and the pure alkaloid indigo obtained from I. suffruticosa on macrophage activation by measuring nitric oxide (NO) and tumour necrosis factor-alpha (TNF-alpha) production. Cytotoxic activity was also evaluated against the two tumour murine cells lines, LM2 (breast adenocarcinoma) and LP07 (lung adenocarcinoma). The alkaloidal fraction induced a high NO production and a moderated TNF-alpha release. The pure indigo demonstrated an elevated NO and TNF-alpha production. The fraction and the pure compound also exhibited cytotoxic activity against both adenocarcinoma cell lines and indigo showed the strongest cytotoxic activity with IC50 value of 0.89 mu g mL(-1) against LM2 and 1.44 mu g mL(-1) against LP07. Our results presented the immunostimulatory and cytotoxic activity of I. suffruticosa, enhancing macrophage function and therefore contributing to the host defence against tumours.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageeng
dc.publisherTaylor & Francis Ltd
dc.relationNatural Product Research
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectalkaloidal fraction
dc.subjectindigo
dc.subjectnitric oxide
dc.subjecttumour necrosis factor-alpha
dc.subjecttumour cell lines
dc.titleImmunostimulatory and cytotoxic activities of Indigofera suffruticosa (Fabaceae)
dc.typeOtro


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