| dc.contributor | Universidade Estadual Paulista (UNESP) | |
| dc.creator | Ferraz, Helen Conceicao | |
| dc.creator | Guimaraes, Juliana Aguilar | |
| dc.creator | Moitinho Alves, Tito Livio | |
| dc.creator | Leopoldo Constantino, Carlos Jose | |
| dc.date | 2014-05-20T13:23:03Z | |
| dc.date | 2016-10-25T16:44:07Z | |
| dc.date | 2014-05-20T13:23:03Z | |
| dc.date | 2016-10-25T16:44:07Z | |
| dc.date | 2011-05-15 | |
| dc.date.accessioned | 2017-04-05T19:56:19Z | |
| dc.date.available | 2017-04-05T19:56:19Z | |
| dc.identifier | Applied Surface Science. Amsterdam: Elsevier B.V., v. 257, n. 15, p. 6535-6539, 2011. | |
| dc.identifier | 0169-4332 | |
| dc.identifier | http://hdl.handle.net/11449/6881 | |
| dc.identifier | http://acervodigital.unesp.br/handle/11449/6881 | |
| dc.identifier | 10.1016/j.apsusc.2011.01.143 | |
| dc.identifier | WOS:000289512500036 | |
| dc.identifier | http://dx.doi.org/10.1016/j.apsusc.2011.01.143 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/855694 | |
| dc.description | Cholesterol oxidase (ChOx) is a flavoenzyme that catalyzes the oxidation of cholesterol to cholest-5-en-3-one and subsequently the isomerization to cholest-4-en-3-one. ChOx has been very commonly studied as the detection element in cholesterol biosensors. In the biosensor development field, a relatively new approach is the use of crystalline bacterial cell surface layers, known as S-Layer proteins. These proteins exhibit the ability of self-assembling at surfaces, opening a vast spectrum of applications, both in basic and applied researches. In our study, monomolecular films of ChOx and mixed films of ChOx/S-Layer proteins and DPPC/S-Layer proteins were produced using the Langmuir technique. Characterization of the films was performed by means of surface pressure-molecular area (pi-A) isotherms. Stable monolayers were obtained, which means that they can be transferred to solid substrates by Langmuir-Blodgett technique. Mixed monolayers showed an ideal like behavior. (C) 2011 Elsevier B. V. All rights reserved. | |
| dc.description | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
| dc.language | eng | |
| dc.publisher | Elsevier B.V. | |
| dc.relation | Applied Surface Science | |
| dc.rights | info:eu-repo/semantics/closedAccess | |
| dc.subject | Cholesterol | |
| dc.subject | Cholesterol oxidase | |
| dc.subject | S-Layer proteins | |
| dc.subject | Monolayer | |
| dc.subject | Biosensor | |
| dc.title | Monomolecular films of cholesterol oxidase and S-Layer proteins | |
| dc.type | Otro | |
