Incorporation of Ag nanoparticles into membrane mimetic systems composed by phospholipid layer-by-layer (LbL) films to achieve surface-enhanced Raman scattering as a tool in drug interaction studies

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorBenites Aoki, Pedro Henrique
dc.creatorAlessio, Priscila
dc.creatorAntonio de Saja, Jose
dc.creatorLeopoldo Constantino, Carlos Jose
dc.date2014-05-20T13:23:02Z
dc.date2016-10-25T16:44:06Z
dc.date2014-05-20T13:23:02Z
dc.date2016-10-25T16:44:06Z
dc.date2010-01-01
dc.date.accessioned2017-04-05T19:56:14Z
dc.date.available2017-04-05T19:56:14Z
dc.identifierJournal of Raman Spectroscopy. Chichester: John Wiley & Sons Ltd, v. 41, n. 1, p. 40-48, 2010.
dc.identifier0377-0486
dc.identifierhttp://hdl.handle.net/11449/6870
dc.identifierhttp://acervodigital.unesp.br/handle/11449/6870
dc.identifier10.1002/jrs.2415
dc.identifierWOS:000274924900007
dc.identifierhttp://dx.doi.org/10.1002/jrs.2415
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/855684
dc.descriptionThe synergistic effect produced by nanoparticles when incorporated into different systems used as analytical tools represents a growing research field nowadays. on the other hand, the study of interactions involving pharmacological drugs and biological membranes using phospholipids as mimetic systems is a research field already well established. Here, we combine both the anionic phospholipid dipalmitoyl phosphatidyl glycerol (DPPG) and negative Ag nanoparticles (AgNP) to form layer-by-layer (LbL) multilayered films using the cationic polymer poly(allylamine hydrochloride) (PAH) as the supporting polyelectrolyte, which were further investigated in the presence of a phenothiazine compound (methylene blue - MB). The molecular architecture of the LbL films in terms of controlled growth, morphology with micro and nanometer spatial resolutions, and dispersion of both AgNP and MB within the DPPG matrix was determined combining spectroscopy [ultraviolet-visible (UV-Vis) absorption and micro-Raman spectroscopy] and microscopy [scanning electron microscopy (SEM) and atomic force microscopy (AFM)]. The results showed that the LbL films can be grown in a controlled way at nanometer thickness scale with the surface morphology susceptible to the presence of both AgNP and MB. The surface-enhanced phenomenon was applied to investigate the LbL films taking the advantage of the strong surface-enhanced resonance Raman scattering (SERRS) signal presented by the MB molecules. Besides, as MB is a pharmacological drug of interest, its molecular arrangements when dispersed in LbL films containing DPPG, which is the biological membrane mimetic system here, were investigated. In this case, the AgNP played a key role in achieving the MB SERRS signal. Copyright (C) 2009 John Wiley & Sons, Ltd.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageeng
dc.publisherJohn Wiley & Sons Ltd
dc.relationJournal of Raman Spectroscopy
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectLbL films
dc.subjectphospholipid
dc.subjectmicro-Raman spectroscopy
dc.subjectSERRS
dc.subjectAFM
dc.titleIncorporation of Ag nanoparticles into membrane mimetic systems composed by phospholipid layer-by-layer (LbL) films to achieve surface-enhanced Raman scattering as a tool in drug interaction studies
dc.typeOtro


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