Alkaloids of Phaedranassa dubia (Kunth) J.F. Macbr. and Phaedranassa brevifolia Meerow (Amaryllidaceae) from Ecuador and its cholinesterase-inhibitory activity

dc.creatorAcosta Le?n, Karen
dc.creatorInca, Alexandra
dc.creatorTallini, Luciana R.
dc.creatorOsorio, Edison H.
dc.creatorRobles, Jessica
dc.creatorBastida, Jaume
dc.creatorOleas, Nora H.
dc.date2022-03-30T16:46:06Z
dc.date2022-03-30T16:46:06Z
dc.date2021-08-04
dc.date.accessioned2023-08-31T19:05:53Z
dc.date.available2023-08-31T19:05:53Z
dc.identifierLe?n, K. A., Inca, A., Tallini, L. R., Osorio, E. H., Robles, J., Bastida, J., & Oleas, N. H. (2021). Alkaloids of Phaedranassa dubia (Kunth) J.F. Macbr. and Phaedranassa brevifolia Meerow (Amaryllidaceae) from Ecuador and its cholinesterase-inhibitory activity. South African Journal of Botany, 136, 91?99. doi:10.1016/j.sajb.2020.09.007
dc.identifier0254-6299
dc.identifierhttps://www.sciencedirect.com/science/article/pii/S0254629920310632
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8555457
dc.descriptionAlzheimer's disease is considered the most common cause of dementia and, in an increasingly aging population worldwide, the quest for treatment is a priority. Amaryllidaceae alkaloids are of main interest because of their cholinesterase inhibition potential, which is the main palliative treatment available for this disease. We evaluated the alkaloidal profile and the in vitro inhibitory activity on acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) of bulb alkaloid extract of Phaedranassa dubia and Phaedranassa brevifolia collected in Ecuador. Using gas chromatography coupled to mass spectrometry (GC-MS), we identified typical Amaryllidaceae alkaloids in these species, highlighting the presence of lycorine-type alkaloids in P. dubia and haemanthamine/crinine-type in P. brevifolia. The species P. dubia and P. brevifolia showed inhibitory activities against AChE (IC50 values of 25.48 ? 0.39 and 3.45 ? 0.29 ?g.mL?1, respectively) and BuChE (IC50 values of 114.96 ? 4.94 and 58.89 ? 0.55 ?g.mL?1, respectively). Computational experiments allowed us to understand the interactions of the alkaloids identified in these samples toward the active sites of AChE and BuChE. In silico, some alkaloids detected in these Amaryllidaceae species presented higher estimated binding free energy toward BuChE than galanthamine. This is the first study about the alkaloid profile and biological potential of P. brevifolia species.
dc.descriptionUniversidad de Ibagu?
dc.languageen
dc.publisherSouth African Journal of Botany
dc.subjectAChE
dc.subjectAlkaloids
dc.subjectAlzheimer's disease
dc.subjectAmaryllidaceae
dc.subjectBuChE
dc.subjectBuChE
dc.subjectPhaedranassa
dc.titleAlkaloids of Phaedranassa dubia (Kunth) J.F. Macbr. and Phaedranassa brevifolia Meerow (Amaryllidaceae) from Ecuador and its cholinesterase-inhibitory activity
dc.typeArticle


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