Detection of mycobacterium avium subsp. paratuberculosis by a direct in situ PCR method

dc.creatorDelgado, Fernando Oscar
dc.creatorAguilar, Diana
dc.creatorGarbaccio, Sergio
dc.creatorFrancinelli, Gladys Beatriz
dc.creatorHernández Pando, R.
dc.creatorRomano, Maria Isabel
dc.date2011-05
dc.date.accessioned2023-08-31T00:42:07Z
dc.date.available2023-08-31T00:42:07Z
dc.identifierhttp://hdl.handle.net/11336/192193
dc.identifierDelgado, Fernando Oscar; Aguilar, Diana; Garbaccio, Sergio; Francinelli, Gladys Beatriz; Hernández Pando, R.; et al.; Detection of mycobacterium avium subsp. paratuberculosis by a direct in situ PCR method; Hindawi Publishing Corporation; Veterinary Medicine International; 2011; 5-2011; 1-5
dc.identifier2042-0048
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8543695
dc.descriptionIn situ detection of Mycobacterium avium subsp. paratuberculosis is useful for diagnosis and research of paratuberculosis. The aim of this paper was to detect this agent in formalin-fixed, paraffin-embedded tissue samples by a direct in situ PCR. The technique was performed on ileum or ileocaecal lymph node samples from 8 naturally infected cattle and 1 healthy calf, by using p89 and p92 primers for amplification of IS900 sequence. Moderate positive signal was detected in all positive samples and not in negative control, but tissues resulted were affected in many cases due to the enzymatic treatment and the high temperature exposition. Although the technique was useful for Map detection, the signal was lower than immunohistochemistry probably because of the fixation process. In one case, signal was higher, which might be due to the detection of spheroplasts. Thus, the described method should be recommended when others resulted negative or for spheroplasts detection.
dc.descriptionFil: Delgado, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
dc.descriptionFil: Aguilar, Diana. Instituto Nacional de Nutricion Salvador Zubiran; México
dc.descriptionFil: Garbaccio, Sergio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
dc.descriptionFil: Francinelli, Gladys Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
dc.descriptionFil: Hernández Pando, R.. Instituto Nacional de Nutricion Salvador Zubiran; México
dc.descriptionFil: Romano, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherHindawi Publishing Corporation
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/vmi/2011/267102/
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/10.4061/2011/267102
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subjectParatuberculosis
dc.subjectPCR
dc.subjectDiagnosis
dc.subjecthttps://purl.org/becyt/ford/4.3
dc.subjecthttps://purl.org/becyt/ford/4
dc.titleDetection of mycobacterium avium subsp. paratuberculosis by a direct in situ PCR method
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


Este ítem pertenece a la siguiente institución