Identification and quantification of a novel nitrate-reducing community in sediments of Suquía River basin along a nitrate gradient

dc.creatorReyna, Luciana
dc.creatorWunderlin, Daniel Alberto
dc.creatorGenti de Raimondi, Susana
dc.date2010-05
dc.date.accessioned2023-08-31T00:38:06Z
dc.date.available2023-08-31T00:38:06Z
dc.identifierhttp://hdl.handle.net/11336/186374
dc.identifierReyna, Luciana; Wunderlin, Daniel Alberto; Genti de Raimondi, Susana; Identification and quantification of a novel nitrate-reducing community in sediments of Suquía River basin along a nitrate gradient; Elsevier; Environmental Pollution; 158; 5; 5-2010; 1608-1614
dc.identifier0269-7491
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8543628
dc.descriptionWe evaluated the molecular diversity of narG gene from Suquía River sediments to assess the impact of the nitrate concentration and water quality on the composition and structure of the nitrate-reducing bacterial community. To this aim, a library of one of the six monitoring stations corresponding to the highest nitrate concentration was constructed and 118 narG clones were screened. Nucleotide sequences were associated to narG gene from alpha-, beta-, delta-, gammaproteobacteria and Thermus thermophilus. Remarkably, 18% of clones contained narG genes with less than 69% similarity to narG sequences available in databases. Thus, indicating the presence of nitrate-reducing bacteria with novel narG genes, which were quantified by real-time PCR. Results show a variable number of narG copies, ranging from less than 1.0 × 102 to 5.0 × 104 copies per ng of DNA, which were associated with a decreased water quality index monitored along the basin at different times.
dc.descriptionFil: Reyna, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina
dc.descriptionFil: Wunderlin, Daniel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina
dc.descriptionFil: Genti de Raimondi, Susana. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0269749109006186
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.envpol.2009.12.014
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subjectNARG GENE
dc.subjectNITRATE GRADIENT
dc.subjectPOLLUTION
dc.subjecthttps://purl.org/becyt/ford/1.6
dc.subjecthttps://purl.org/becyt/ford/1
dc.titleIdentification and quantification of a novel nitrate-reducing community in sediments of Suquía River basin along a nitrate gradient
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


Este ítem pertenece a la siguiente institución