Genetic and biochemical biomarkers in the macrophyte Bidens laevis L. exposed to a commercial formula of endosulfan

dc.creatorPérez, Débora Jesabel
dc.creatorLukaszewicz, Germán
dc.creatorMenone, Mirta Lujan
dc.creatorAmé, María Valeria
dc.creatorCamadro, Elsa Lucila
dc.date2014-01
dc.date.accessioned2023-08-31T00:35:23Z
dc.date.available2023-08-31T00:35:23Z
dc.identifierhttp://hdl.handle.net/11336/180487
dc.identifierPérez, Débora Jesabel; Lukaszewicz, Germán; Menone, Mirta Lujan; Amé, María Valeria; Camadro, Elsa Lucila; Genetic and biochemical biomarkers in the macrophyte Bidens laevis L. exposed to a commercial formula of endosulfan; John Wiley & Sons Inc.; Environmental Toxicology; 29; 9; 1-2014; 1063-1071
dc.identifier1520-4081
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8543585
dc.descriptionPrevious studies in the wetland macrophyte Bidens laevis L have demonstrated that the insecticide endosulfan induces a high frequency of somatic chromosome aberrations in anaphase-telophase (CAAT) but no DNA changes as determined by the single cell gel electrophoresis (Comet) assay. Thus, cytogenetic biomarkers appear to be more sensitive to the toxic effects of the insecticide than the DNA molecule in the studied species. For this reason, the goals of this study were to use cytogenetic biomarkers-CAAT and abnormal metaphase-and defense biomarkers such as the activity of the antioxidant enzymes-guaiacol peroxidases (POD), glutathione reductase, and microsomal and cytosolic (m- and c-) glutathione-S-transferase (GST)-to evaluate in B. laevis effects caused by a commercial formulation of endosulfan. The frequency of CAAT was increased at 5, 10, 50, and 100 μg/L endosulfan with respect to the negative controls by 3.1, 2.5, 2.5, and 3.2-fold, respectively while the frequency of abnormal metaphases was also increased at the same concentrations by 3.5, 2.8, 3.2, and 11.3-fold, respectively. In addition to these aneugenic effects, other abnormalities such as C-mitosis and chromosome clumping were observed at 10 μg/L endosulfan. On the other hand, POD induction at 0.02, 0.5, 5, and 10 μg/L and m-GST inhibition at 0.5, 10, and 50 μg/L in plants exposed during 24 h to endosulfan were observed but all of these responses were highly variable. In conclusion, only cytogenetic biomarkers like CAAT in B. laevis can serve potentially as early warning systems to detect environmentally relevant concentrations of endosulfan in aquatic ecosystems.
dc.descriptionFil: Pérez, Débora Jesabel. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Ciencias Marinas. Laboratorio de Ecotoxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
dc.descriptionFil: Lukaszewicz, Germán. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Ciencias Marinas. Laboratorio de Ecotoxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
dc.descriptionFil: Menone, Mirta Lujan. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Ciencias Marinas. Laboratorio de Ecotoxicología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
dc.descriptionFil: Amé, María Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba; Argentina
dc.descriptionFil: Camadro, Elsa Lucila. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
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dc.languageeng
dc.publisherJohn Wiley & Sons Inc.
dc.relationinfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/tox.21836
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/10.1002/tox.21836
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subjectCHROMOSOME ABERRATIONS
dc.subjectCOMMERCIAL FORMULATION OF ENDOSULFAN
dc.subjectGENOTOXICITY
dc.subjectPEROXIDASES
dc.subjectWETLAND MACROPHYTES
dc.subjecthttps://purl.org/becyt/ford/1.5
dc.subjecthttps://purl.org/becyt/ford/1
dc.titleGenetic and biochemical biomarkers in the macrophyte Bidens laevis L. exposed to a commercial formula of endosulfan
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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