TSSK3, a novel target for male contraception, is required for spermiogenesis

dc.creatorNayyab, Saman
dc.creatorGervasi, María G.
dc.creatorTourzani, Darya A.
dc.creatorCaraballo, Diego Alfredo
dc.creatorJha, Kula N.
dc.creatorTeves, Maria E.
dc.creatorCui, Wei
dc.creatorGeorg, Gunda I.
dc.creatorVisconti, Pablo E.
dc.creatorSalicioni, Ana M.
dc.date2021-10
dc.date.accessioned2023-08-31T00:28:43Z
dc.date.available2023-08-31T00:28:43Z
dc.identifierhttp://hdl.handle.net/11336/181461
dc.identifierNayyab, Saman; Gervasi, María G.; Tourzani, Darya A.; Caraballo, Diego Alfredo; Jha, Kula N.; et al.; TSSK3, a novel target for male contraception, is required for spermiogenesis; Wiley-liss, div John Wiley & Sons Inc.; Molecular Reproduction and Development; 88; 11; 10-2021; 718-730
dc.identifier1040-452X
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8543463
dc.descriptionWe have previously shown that members of the family of testis-specific serine/threonine kinases (TSSKs) are post-meiotically expressed in testicular germ cells and in mature sperm in mammals. The restricted post-meiotic expression of TSSKs as well as the importance of phosphorylation in signaling processes strongly suggest that TSSKs have an important role in germ cell differentiation and/or sperm function. This prediction has been supported by the reported sterile phenotype of the TSSK6 knock-out (KO) mice and of the double TSSK1/TSSK2 KO. The aim of this study was to develop KO mouse models of TSSK3 and to validate this kinase as a target for the development of a male contraceptive. We used CRISPR/Cas9 technology to generate the TSSK3 KO allele on B6D2F1 background mice. Male heterozygous pups were used to establish three independent TSSK3 KO lines. After natural mating of TSSK3 KO males, females that presented a plug (indicative of mating) were monitored for the following 24 days and no pregnancies or pups were found. Sperm numbers were drastically reduced in all three KO lines and, remarkably, round spermatids were detected in the cauda epididymis of KO mice. From the small population of sperm recovered, severe morphology defects were detected. Our results indicate an essential role of TSSK3 in spermiogenesis and support this kinase as a suitable candidate for the development of novel nonhormonal male contraceptives.
dc.descriptionFil: Nayyab, Saman. University of Massachussets; Estados Unidos
dc.descriptionFil: Gervasi, María G.. University of Massachussets; Estados Unidos
dc.descriptionFil: Tourzani, Darya A.. University of Massachussets; Estados Unidos
dc.descriptionFil: Caraballo, Diego Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
dc.descriptionFil: Jha, Kula N.. No especifíca;
dc.descriptionFil: Teves, Maria E.. University of Virginia; Estados Unidos
dc.descriptionFil: Cui, Wei. University of Massachussets; Estados Unidos
dc.descriptionFil: Georg, Gunda I.. University of Minnesota; Estados Unidos
dc.descriptionFil: Visconti, Pablo E.. University of Massachussets; Estados Unidos
dc.descriptionFil: Salicioni, Ana M.. University of Massachussets; Estados Unidos
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherWiley-liss, div John Wiley & Sons Inc.
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/mrd.23539
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/10.1002/mrd.23539
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subjectEVOLUTION
dc.subjectFERTILIZATION
dc.subjectINTRONLESS GENE
dc.subjectKINASES
dc.subjectNONHORMONAL MALE CONTRACEPTIVE
dc.subjectSPERM
dc.subjectSPERMATOGENESIS
dc.subjectTESTIS-SPECIFIC
dc.subjectTSSK3
dc.subjecthttps://purl.org/becyt/ford/1.6
dc.subjecthttps://purl.org/becyt/ford/1
dc.subjecthttps://purl.org/becyt/ford/1.6
dc.subjecthttps://purl.org/becyt/ford/1
dc.subjecthttps://purl.org/becyt/ford/1.6
dc.subjecthttps://purl.org/becyt/ford/1
dc.titleTSSK3, a novel target for male contraception, is required for spermiogenesis
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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