Detection of Shiga toxin producing Escherichia coli in endocervix of asymptomatic pregnant women: Novel pathogen responsible for adverse pregnancy outcomes?

dc.creatorScalise, Maria Lujan
dc.creatorGarimano, Nicolás Ezequiel
dc.creatorPorporato, Melina Mabel
dc.creatorLeonino, Patricia
dc.creatorPereyra, Adriana
dc.creatorFerreiros, José A.
dc.creatorCasale, Roberto
dc.creatorAmaral, María Marta
dc.creatorIbarra, Cristina Adriana
dc.creatorSacerdoti, Flavia
dc.date2021
dc.date.accessioned2023-08-31T00:11:04Z
dc.date.available2023-08-31T00:11:04Z
dc.identifierhttp://hdl.handle.net/11336/193057
dc.identifierDetection of Shiga toxin producing Escherichia coli in endocervix of asymptomatic pregnant women: Novel pathogen responsible for adverse pregnancy outcomes?; International Symposium on Reproductive Health; Virtual Meeting; Singapur; 2021; 742-743
dc.identifier2709-0094
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8543189
dc.descriptionBrief Introduction: Some studies have demonstrated that vaginal E. coli colonization may produce complicationsduring pregnancy. We have previously reported that Shiga toxin-2 (Stx2) secreted by enterohemorrhagic E. coli canproduce abortion and premature delivery in animals and can impair human trophoblast in vitro.Objective: The aim of this study was to detect virulence factor genes from Shiga toxin producing E. coli (STEC)in the endocervix of asymptomatic pregnant women. Methods: Endocervical swabs were collected from 103 pregnantwomen (12 to 30 weeks of pregnancy) during their antenatal examination. Swab samples were enriched in TrypticSoy Broth overnight at 37 °C and then streaked into selective and differential medium, Sorbitol MacConkey agar. E.coli detection was confirmed by identification of uidA gene by PCR assay. Then, positive samples for E. coli wereanalyzed for STEC virulence factors genes: stx1, stx2, eae, rfbO157, lpfAO113 and hcpA. After that, positive E. colisamples for stx2 gene were grown in Luria-Bertani Broth medium in order to evaluate Stx2 cytotoxic activity. Forthat, the bacterial supernatants were filter-sterilized and cytotoxicity was evaluated on Vero, Swan 71 and HeLa cells.Results: Our results showed that 14.5% of the endocervical samples were positive for E. coli (positive for uidAgene). Furthermore, 9/15 (60%) of the E. coli-positive samples carried the stx2 gene and 6/15 (40%) carried thelpfAO113 and hcpA genes. One bacterial supernatant from an E. coli-positive endocervical sample carrying stx2 andlpfAO113 genes exhibited high cytotoxic activity on the cells evaluated due to Stx2 production confirmed by specificneutralization of Stx2 with an anti-Stx2 antibody.Conclusions: The novelty of this report is the presence of STEC in the endocervix of asymptomatic pregnantwomen. This opens a new perspective with respect to the possible role of this pathogen in woman reproductivehealth care.
dc.descriptionFil: Scalise, Maria Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
dc.descriptionFil: Garimano, Nicolás Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
dc.descriptionFil: Porporato, Melina Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
dc.descriptionFil: Leonino, Patricia. Hospital Nacional Profesor Alejandro Posadas.; Argentina
dc.descriptionFil: Pereyra, Adriana. Hospital Nacional Profesor Alejandro Posadas.; Argentina
dc.descriptionFil: Ferreiros, José A.. Hospital Nacional Profesor Alejandro Posadas.; Argentina
dc.descriptionFil: Casale, Roberto. Hospital Nacional Profesor Alejandro Posadas.; Argentina
dc.descriptionFil: Amaral, María Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
dc.descriptionFil: Ibarra, Cristina Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
dc.descriptionFil: Sacerdoti, Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
dc.descriptionInternational Symposium on Reproductive Health
dc.descriptionVirtual Meeting
dc.descriptionSingapur
dc.descriptionThe Physicologycal Society
dc.descriptionBiochemical Society
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherIMR Press
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://imrpress.com/journal/CEOG/48/3/10.31083/j.ceog.2021.03.0511
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subjectHUS
dc.subjectPregnancy
dc.subjectStx2
dc.subjecthttps://purl.org/becyt/ford/3.3
dc.subjecthttps://purl.org/becyt/ford/3
dc.titleDetection of Shiga toxin producing Escherichia coli in endocervix of asymptomatic pregnant women: Novel pathogen responsible for adverse pregnancy outcomes?
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typeinfo:eu-repo/semantics/conferenceObject
dc.typeinfo:ar-repo/semantics/documento de conferencia
dc.typeSimposio
dc.typeJournal
dc.coverageInternacional


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