Nucleolar release of rDNA repeats for repair involves SUMO-mediated untethering by the Cdc48/p97 segregase

dc.creatorCapella, Matias
dc.creatorMandemaker, Imke K.
dc.creatorMartín Caballero, Lucía
dc.creatorden Brave, Fabian
dc.creatorPfander, Boris
dc.creatorLadurner, Andreas G.
dc.creatorJentsch, Stefan
dc.creatorBraun, Sigurd
dc.date2021-08
dc.date.accessioned2023-08-31T00:08:19Z
dc.date.available2023-08-31T00:08:19Z
dc.identifierhttp://hdl.handle.net/11336/183992
dc.identifierCapella, Matias; Mandemaker, Imke K.; Martín Caballero, Lucía; den Brave, Fabian; Pfander, Boris; et al.; Nucleolar release of rDNA repeats for repair involves SUMO-mediated untethering by the Cdc48/p97 segregase; Nature Publishing Group; Nature Communications; 12; 1; 8-2021; 1-16
dc.identifier2041-1723
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8543136
dc.descriptionRibosomal RNA genes (rDNA) are highly unstable and susceptible to rearrangement due to their repetitive nature and active transcriptional status. Sequestration of rDNA in the nucleolus suppresses uncontrolled recombination. However, broken repeats must be first released to the nucleoplasm to allow repair by homologous recombination. Nucleolar release of broken rDNA repeats is conserved from yeast to humans, but the underlying molecular mechanisms are currently unknown. Here we show that DNA damage induces phosphorylation of the CLIP-cohibin complex, releasing membrane-tethered rDNA from the nucleolus in Saccharomyces cerevisiae. Downstream of phosphorylation, SUMOylation of CLIP-cohibin is recognized by Ufd1 via its SUMO-interacting motif, which targets the complex for disassembly through the Cdc48/p97 chaperone. Consistent with a conserved mechanism, UFD1L depletion in human cells impairs rDNA release. The dynamic and regulated assembly and disassembly of the rDNA-tethering complex is therefore a key determinant of nucleolar rDNA release and genome integrity.
dc.descriptionFil: Capella, Matias. Max Planck Institute Of Biochemistry.; Alemania. Ludwig Maximilians Universitat; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.descriptionFil: Mandemaker, Imke K.. Ludwig Maximilians Universitat; Alemania
dc.descriptionFil: Martín Caballero, Lucía. Ludwig Maximilians Universitat; Alemania. International Max Planck Research School For Molecular Life Sciences: From Biological Structures To Neural Circuits; Alemania
dc.descriptionFil: den Brave, Fabian. Max-planck-institut Für Biochemie; Alemania. Universitat Bonn; Alemania
dc.descriptionFil: Pfander, Boris. International Max Planck Research School For Molecular Life Sciences: From Biological Structures To Neural Circuits; Alemania. Max-planck-institut Für Biochemie; Alemania
dc.descriptionFil: Ladurner, Andreas G.. International Max Planck Research School For Molecular Life Sciences: From Biological Structures To Neural Circuits; Alemania. Ludwig Maximilians Universitat; Alemania
dc.descriptionFil: Jentsch, Stefan. Max-planck-institut Für Biochemie; Alemania
dc.descriptionFil: Braun, Sigurd. Ludwig Maximilians Universitat; Alemania. Justus-liebig-universität Giessen; Alemania. International Max Planck Research School For Molecular Life Sciences: From Biological Structures To Neural Circuits; Alemania
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherNature Publishing Group
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41467-021-25205-2
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41467-021-25205-2
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subjectRibosomal DNA
dc.subjectGenome stability
dc.subjectDNA damage
dc.subjectSaccharomyces cerevisiae
dc.subjecthttps://purl.org/becyt/ford/1.6
dc.subjecthttps://purl.org/becyt/ford/1
dc.titleNucleolar release of rDNA repeats for repair involves SUMO-mediated untethering by the Cdc48/p97 segregase
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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