Tumor extracellular matrix: lessons from the second-harmonic generation microscopy

dc.creatorde Andrade Natal, Rodrigo
dc.creatorAdur, Javier Fernando
dc.creatorLenz Cesar, Carlos
dc.creatorVassallo, José
dc.date2021-03
dc.date.accessioned2023-08-30T23:47:56Z
dc.date.available2023-08-30T23:47:56Z
dc.identifierhttp://hdl.handle.net/11336/173415
dc.identifierde Andrade Natal, Rodrigo; Adur, Javier Fernando; Lenz Cesar, Carlos; Vassallo, José; Tumor extracellular matrix: lessons from the second-harmonic generation microscopy; Springer; Surgical and Experimental Pathology; 4; 1; 3-2021; 1-12
dc.identifier2520-8454
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8542791
dc.descriptionExtracellular matrix (ECM) represents more than a mere intercellular cement. It is physiologically active in cell communication, adhesion and proliferation. Collagen is the most abundant protein, making up to 90% of ECM, and 30% of total protein weight in humans. Second-harmonic generation (SHG) microscopy represents an important tool to study collagen organization of ECM in freshly unfixed tissues and paraffin-embedded tissue samples. This manuscript aims to review some of the applications of SHG microscopy in Oncologic Pathology, mainly in the study of ECM of epithelial tumors. It is shown how collagen parameters measured by this technique can aid in the differential diagnosis and in prognostic stratification. There is a tendency to associate higher amount, lower organization and higher linearity of collagen fibers with tumor progression and metastasizing. These represent complex processes, in which matrix remodeling plays a central role, together with cancer cell genetic modifications. Integration of studies on cancer cell biology and ECM are highly advantageous to give us a more complete picture of these processes. As microscopic techniques provide topographic information allied with biologic characteristics of tissue components, they represent important tools for a more complete understanding of cancer progression. In this context, SHG has provided significant insights in human tumor specimens, readily available for Pathologists.
dc.descriptionFil: de Andrade Natal, Rodrigo. Universidade Estadual de Campinas; Brasil
dc.descriptionFil: Adur, Javier Fernando. Universidad Nacional de Entre Ríos. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación y Desarrollo en Bioingeniería y Bioinformática; Argentina
dc.descriptionFil: Lenz Cesar, Carlos. Universidade Estadual de Campinas; Brasil
dc.descriptionFil: Vassallo, José. Universidade Estadual de Campinas; Brasil
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherSpringer
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/10.1186/s42047-021-00089-0
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttps://creativecommons.org/licenses/by/2.5/ar/
dc.subjectMatriz Extracelular
dc.subjectCáncer
dc.subjectMicroscopia No lineal
dc.subjectSHG
dc.subjecthttps://purl.org/becyt/ford/1.6
dc.subjecthttps://purl.org/becyt/ford/1
dc.titleTumor extracellular matrix: lessons from the second-harmonic generation microscopy
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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