Small-angle X-ray scattering to quantify the incorporation and analyze the disposition of magnetic nanoparticles inside cells

dc.creatorCoral, Diego Fernando
dc.creatorSoto, Paula Andrea
dc.creatorde Sousa, Maria Elisa
dc.creatorBrollo, M. E. F.
dc.creatorMera-Córdoba, J. A.
dc.creatorMendoza Zélis, Pedro
dc.creatorSetton, Clara Patricia
dc.creatorRoig, A.
dc.creatorFernández van Raap, Marcela Beatriz
dc.date2022-02
dc.date.accessioned2023-08-30T23:47:25Z
dc.date.available2023-08-30T23:47:25Z
dc.identifierhttp://hdl.handle.net/11336/181189
dc.identifierCoral, Diego Fernando; Soto, Paula Andrea; de Sousa, Maria Elisa; Brollo, M. E. F.; Mera-Córdoba, J. A.; et al.; Small-angle X-ray scattering to quantify the incorporation and analyze the disposition of magnetic nanoparticles inside cells; Academic Press Inc Elsevier Science; Journal of Colloid and Interface Science; 608; 2-2022; 1-12
dc.identifier0021-9797
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8542781
dc.descriptionAccess to detailed information on cells loaded with nanoparticles with nanoscale precision is of a long-standing interest in many areas of nanomedicine. In this context, designing a single experiment able to provide statistical mean data from a large number of living unsectioned cells concerning information on the nanoparticle size and aggregation inside cell endosomes and accurate nanoparticle cell up-take is of paramount importance. Small-angle X-ray scattering (SAXS) is presented here as a tool to achieve such relevant data. Experiments were carried out in cultures of B16F0 murine melanoma and A549 human lung adenocarcinoma cell lines loaded with various iron oxide nanostructures displaying distinctive structural characteristics. Five systems of water-dispersible magnetic nanoparticles (MNP) of different size, polydispersity and morphology were analyzed, namely, nearly monodisperse MNP with 11 and 13 nm mean size coated with meso-2,3-dimercaptosuccinic acid, more polydisperse 6 nm colloids coated with citric acid and two nanoflowers (NF) systems of 24 and 27 nm in size resulting from the aggregation of 8 nm MNP. Up-take was determined for each system using B16F0 cells. Here we show that SAXS pattern provides high resolution information on nanoparticles disposition inside endosomes of the cytoplasm through the structure factor analysis, on nanoparticles size and dispersity after their incorporation by the cell and on up-take quantification from the extrapolation of the intensity in absolute scale to null scattering vector. We also report on the cell culture preparation to reach sensitivity for the observation of MNP inside cell endosomes using high brightness SAXS synchrotron source. Our results show that SAXS can become a valuable tool for analyzing MNP in cells and tissues.
dc.descriptionFil: Coral, Diego Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física La Plata; Argentina. Universidad del Cauca; Colombia
dc.descriptionFil: Soto, Paula Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
dc.descriptionFil: de Sousa, Maria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física La Plata; Argentina
dc.descriptionFil: Brollo, M. E. F.. Instituto de Ciencia de Materiales de Madrid; España
dc.descriptionFil: Mera-Córdoba, J. A.. Institución Universitaria Cesmag; Colombia
dc.descriptionFil: Mendoza Zélis, Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física La Plata; Argentina
dc.descriptionFil: Setton, Clara Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
dc.descriptionFil: Roig, A.. Consejo Superior de Investigaciones Científicas. Instituto de Ciencia de los Materiales de Barcelona; España
dc.descriptionFil: Fernández van Raap, Marcela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física La Plata; Argentina
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherAcademic Press Inc Elsevier Science
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0021979721016301
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jcis.2021.09.165
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subjectA549, B16F0, CELL UP-TAKE
dc.subjectIRON OXIDE
dc.subjectNANOFLOWERS
dc.subjectSAXS
dc.subjectSMALL ANGLE X-RAY SCATTERING
dc.subjectSUPERPARAMAGNETIC NANOPARTICLES
dc.subjectSYNCHROTRON RADIATION, SOFT MATER
dc.subjecthttps://purl.org/becyt/ford/2.10
dc.subjecthttps://purl.org/becyt/ford/2
dc.titleSmall-angle X-ray scattering to quantify the incorporation and analyze the disposition of magnetic nanoparticles inside cells
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


Este ítem pertenece a la siguiente institución