Effects of NGF Addition on Llama (Lama glama) Sperm Traits After Cooling

dc.creatorSari, Luciana María
dc.creatorZampini, Renato
dc.creatorGonzalez del Pino, Francisco
dc.creatorArgañaraz, Martin Eduardo
dc.creatorRatto, Marcelo Hector
dc.creatorApichela, Silvana Andrea
dc.date2021-01
dc.date.accessioned2023-08-30T23:39:43Z
dc.date.available2023-08-30T23:39:43Z
dc.identifierhttp://hdl.handle.net/11336/184307
dc.identifierSari, Luciana María; Zampini, Renato; Gonzalez del Pino, Francisco; Argañaraz, Martin Eduardo; Ratto, Marcelo Hector; et al.; Effects of NGF Addition on Llama (Lama glama) Sperm Traits After Cooling; Frontiers Media; Frontiers in Veterinary Science; 7; 1-2021; 1-8
dc.identifier2297-1769
dc.identifierCONICET Digital
dc.identifierCONICET
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8542658
dc.descriptionTo provide new insights into the mechanisms through which seminal plasma proteins can protect sperm from damage caused during refrigeration, we evaluate the possibility that β-NGF can contribute to the improvement of sperm quality after cooling. First, β-NGF was detected in refrigerated sperm and compared with unrefrigerated sperm by western blotting of the proteins adsorbed by sperm, showing that native β-NGF is still present even 24 h after cooling only as an active form. Then, the effect of exogenous β-NGF on sperm quality after cooling was evaluated. A total of 12 ejaculates from male llamas (three ejaculates per male), were obtained by electro-ejaculation, diluted 4:1 with buffer Hepes-balanced salt solution and centrifuged at 800 × g for 8 min to remove the seminal plasma. Sperm were suspended in Tris-citrate-fructose-egg yolk diluent for a final concentration of 30 ×106/ml and cooled at 5°C for 24 h. After refrigeration, the extended sperm were equilibrated for 5 min at 37°C and divided into the following subgroups: sperm samples without treatment (control) and sperm samples supplemented with exogenous human β-NGF (10, 100, and 500 ng/ml). At 5, 30, and 60 min of incubation sperm were evaluated for sperm viability (using eosin/nigrosin stain), sperm motility and vigor (observed under light microscopy), and mitochondrial activity (using the JC-1 fluorescent marker). Vigor data were analyzed with the nonparametric Kruskal-Wallis test. The rest of the variables were analyzed with a mixed models approach. Mean comparisons were performed using Fisher's LSD test with a confidence level of 95%. A principal components analysis was performed to analyze the relationships between variables. Treatment of 24 h cooled sperm with 10 or 100 ng/ml of human β-NGF increased the percentage of total motility and vigor (p < 0.05). Besides, an incubation time of 60 min would be adequate to improve sperm quality, since all variables are positively related. The significant improvement observed in the motility and vigor of post-refrigerated sperm suggests that supplementation with exogenous β-NGF may be profitable for the improvement of cooled llama sperm.
dc.descriptionFil: Sari, Luciana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
dc.descriptionFil: Zampini, Renato. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
dc.descriptionFil: Gonzalez del Pino, Francisco. Universidad Nacional de Tucumán; Argentina
dc.descriptionFil: Argañaraz, Martin Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
dc.descriptionFil: Ratto, Marcelo Hector. Universidad Austral de Chile; Chile
dc.descriptionFil: Apichela, Silvana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
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dc.languageeng
dc.publisherFrontiers Media
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fvets.2020.610597/full
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fvets.2020.610597
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subjectCOOLING
dc.subjectCRYOPRESERVATION
dc.subjectLLAMA (LAMA GLAMA)
dc.subjectSEMINAL PLASMA
dc.subjectSPERM
dc.subjectΒ-NERVE GROWTH FACTOR
dc.subjecthttps://purl.org/becyt/ford/4.2
dc.subjecthttps://purl.org/becyt/ford/4
dc.titleEffects of NGF Addition on Llama (Lama glama) Sperm Traits After Cooling
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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