The draft genome of Andean Rhodopseudomonas sp. strain AZUL predicts genome plasticity and adaptation to chemical homeostasis

dc.creatorGuardia, Aisha E.
dc.creatorWagner, Agustín
dc.creatorBusalmen, Juan P.
dc.creatorDi Capua, Cecilia Beatriz
dc.creatorCortez, Néstor
dc.creatorBeligni, María V.
dc.date2023-02-06T15:01:24Z
dc.date2023-02-06T15:01:24Z
dc.date2022-12-09
dc.date2023-02-06T15:01:24Z
dc.date2023-02-06T15:01:24Z
dc.date2022-12-09
dc.date.accessioned2023-08-30T21:17:46Z
dc.date.available2023-08-30T21:17:46Z
dc.identifier1471-2180
dc.identifierhttp://hdl.handle.net/2133/25134
dc.identifierhttp://hdl.handle.net/2133/25134
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8542013
dc.descriptionThe genus Rhodopseudomonas comprises purple non-sulfur bacteria with extremely versatile metabolisms. Characterization of several strains revealed that each is a distinct ecotype highly adapted to its specific micro-habitat. Here we present the sequencing, genomic comparison and functional annotation of AZUL, a Rhodopseudomonas strain isolated from a high altitude Andean lagoon dominated by extreme conditions and fluctuating levels of chemicals. Average nucleotide identity (ANI) analysis of 39 strains of this genus showed that the genome of AZUL is 96.2% identical to that of strain AAP120, which suggests that they belong to the same species. ANI values also show clear separation at the species level with the rest of the strains, being more closely related to R. palustris. Pangenomic analyses revealed that the genus Rhodopseudomonas has an open pangenome and that its core genome represents roughly 5 to 12% of the total gene repertoire of the genus. Functional annotation showed that AZUL has genes that participate in conferring genome plasticity and that, in addition to sharing the basal metabolic complexity of the genus, it is also specialized in metal and multidrug resistance and in responding to nutrient limitation. Our results also indicate that AZUL might have evolved to use some of the mechanisms involved in resistance as redox reactions for bioenergetic purposes. Most of those features are shared with strain AAP120, and mainly involve the presence of additional orthologs responsible for the mentioned processes. Altogether, our results suggest that AZUL, one of the few bacteria from its habitat with a sequenced genome, is highly adapted to the extreme and changing conditions that constitute its niche.
dc.descriptionFil: Guardia, Aisha E. Ingeniería de Interfases y Bioprocesos. Instituto de Tecnología de Materiales (INTEMA-CONICET-UNMdP); Argentina.
dc.descriptionFil: Busalmen, Juan P. Ingeniería de Interfases y Bioprocesos. Instituto de Tecnología de Materiales (INTEMA-CONICET-UNMdP); Argentina.
dc.descriptionFil: Wagner, Agustín. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.
dc.descriptionFil: Di Capua, Cecilia Beatriz. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET-UNR); Argentina.
dc.descriptionFil: Cortez, Néstor. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET-UNR); Argentina.
dc.descriptionFil: Beligni, María V. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas (IIB-CONICET-UNMdP); Argentina.
dc.formatapplication/pdf
dc.languageeng
dc.publisherBMC
dc.relationhttps://doi.org/10.1186/s12866-022-02685-w
dc.relationhttps://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-022-02685-w#article-info
dc.rightshttp://creativecommons.org/licenses/by/4.0/
dc.rightsGuardia, Aisha E.
dc.rightsWagner, Agustín
dc.rightsBusalmen, Juan P.
dc.rightsDi Capua, Cecilia Beatriz
dc.rightsCortez, Néstor
dc.rightsBeligni, María V.
dc.rightsAttribution 4.0 International (CC BY 4.0)
dc.rightsopenAccess
dc.subjectChemical resistance
dc.subjectHigh-altitude Andean lakes
dc.subjectPangenomic analysis
dc.subjectPurple non-sulfur bacteria
dc.subjectRhodopseudomonas
dc.titleThe draft genome of Andean Rhodopseudomonas sp. strain AZUL predicts genome plasticity and adaptation to chemical homeostasis
dc.typearticle
dc.typeartículo
dc.typepublishedVersion


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