| dc.creator | Nicolas, Juan Carlos | |
| dc.creator | Grumelli, Yanina Alejandra | |
| dc.date | 2004-03-31 | |
| dc.date.accessioned | 2023-08-30T17:09:16Z | |
| dc.date.available | 2023-08-30T17:09:16Z | |
| dc.identifier | http://pa.bibdigital.ucc.edu.ar/3982/1/A_Grumelli_Nicolas.pdf | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8539711 | |
| dc.description | Almost twenty years ago a new type of autoimmune chronic hepatitis, denominated Type 2 Autoimmune Hepatitis was described. It is more frequent in girls and young women, characterized by the presence of serum autoantibodies liver/kidney anti microsomes type 1 (LKM-1). A microsomic enzyme of 50 kDa, the Cytochrome P450 Monooxygenase (P450IID6) metabolizes drugs and is the target antigen of the LKM-1. This antigen can also be expressed in the cellular membrane of the hepatocytes modulated by interleukines and tumoral necrosis factor (TNF). The most frequent method to determine antibodies anti LKM-1 is the indirect immunofluorescence, using liver and kidney rat slides. The main inconvenience is the similar pattern that they present with other antibodies denominated Antimitochondrial (AMA). In this work, a test for LKM is developed using an Enzime-linked Immunosorbent Assay (ELISA), starting from extracted liver rat microsomes. The Interassay Variation Coefficients for two significance levels, both negative and positive-LKM samples were respectively 12.5% and 8.7%. Sensibility and specificity were 100% and 97.5% respectively for both samples. | |
| dc.description | Fil: Nicolas, Juan Carlos. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina | |
| dc.description | Fil: Grumelli, Yanina Alejandra. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina | |
| dc.format | application/pdf | |
| dc.language | spa | |
| dc.publisher | Federación Bioquímica de la Provincia de Buenos Aires | |
| dc.relation | http://pa.bibdigital.ucc.edu.ar/3982/ | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.rights | https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es | |
| dc.source | Nicolas, Juan Carlos ORCID: https://orcid.org/0000-0001-9163-2668 <https://orcid.org/0000-0001-9163-2668> and Grumelli, Yanina Alejandra ORCID: https://orcid.org/0000-0001-6234-865X <https://orcid.org/0000-0001-6234-865X> (2004) ELISA method for assess liver - Kidney microsomes antibodies [Método ELISA para anticuerpos antimicrosomales hígado-riñón]. Acta Bioquímica Clínica Latinoamericana, 38 (1). pp. 23-27. ISSN 0325-2957 | |
| dc.subject | QD Química | |
| dc.title | ELISA method for assess liver - Kidney microsomes antibodies
[Método ELISA para anticuerpos antimicrosomales hígado-riñón] | |
| dc.type | info:eu-repo/semantics/article | |
| dc.type | info:ar-repo/semantics/artículo | |
| dc.type | info:eu-repo/semantics/acceptedVersion | |
