Otro
Prevalence and Molecular Characterization of Mycoplasma ovis in Selected Free-Ranging Brazilian Deer Populations
Registro en:
Journal of Wildlife Diseases. Lawrence: Wildlife Disease Assoc, Inc, v. 47, n. 4, p. 1005-1011, 2011.
0090-3558
WOS:000296409500025
Autor
Grazziotin, Ana Laura
Barbanti Duarte, Jose Mauricio
Juan Szabo, Matias Pablo
Santos, Andrea Pires
Sa Guimaraes, Ana Marcia
Mohamed, Ahmed
da Costa Vieira, Rafael Felipe
de Barros Filho, Ivan Roque
Biondo, Alexander Welker
Messick, Joanne Belle
Resumen
Mycoplasma ovis is a hemoplasma that may cause anemia and mortality in small ruminants. Our aim was to determine whether M. ovis infects populations of free-ranging deer in Brazil. Bully coat samples from 64 Blastocerus dichotomus from Porto Primavera, 18 Ozotocerus bezoarticus from Pantanal, and 21 O. bezoarticus from Emas National Park were tested. Using a M. ovis PCR protocol to amplify extracted DNA, 46/64 (72%) of deer froth Porto Primavera, 10/18 (56%) from Pantanal, and 4/21 (19%) from Emas National Park were positive, giving an overall positive rate of 58% for hemoplasma in these wild deer. Sequencing and phylogenetic analysis of the 168 rRNA gene revealed 3 genetically distinct hemoplasmas including M. ovis, 'Candidatus Mycoplasma erythrocervae', and a hemoplasma most closely related to M. ovis. Phylogenetic analysis of the 23S rRNA gene from selected sequences confirmed these relationships.