Dengue Virus Uses a Non-Canonical Function of the Host GBF1-Arf-COPI System for Capsid Protein Accumulation on Lipid Droplets

dc.creatorIglesias, Nestor G.
dc.creatorMondotte, Juan A.
dc.creatorByk, Laura A.
dc.creatorDe Maio, Federico Andrés
dc.creatorSamsa, Marcelo M.
dc.creatorAlvarez, Cecilia I.
dc.creatorGamarnik, Andrea V.
dc.date2015-06
dc.date.accessioned2023-08-30T16:38:34Z
dc.date.available2023-08-30T16:38:34Z
dc.identifierIglesias, Nestor G., Mondotte, Juan A., Byk, Laura A., de Maio, Federico A., Samsa, Marcelo M., et al. (2015). Dengue Virus Uses a Non-Canonical Function of the Host GBF1-Arf-COPI System for Capsid Protein Accumulation on Lipid Droplets. Wiley; Traffic; 16; 9; pp. 962-977
dc.identifier1398-9219
dc.identifierhttp://onlinelibrary.wiley.com/doi/10.1111/tra.12305/abstract
dc.identifierhttp://hdl.handle.net/11336/7808
dc.identifierhttps://rid.unrn.edu.ar/jspui/handle/20.500.12049/2632
dc.identifierhttp://dx.doi.org/10.1111/tra.12305
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8534123
dc.descriptionFil: Iglesias, Nestor G. Fundación Instituto Leloir; Argentina.
dc.descriptionFil: Iglesias, Nestor G. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.descriptionFil: Mondotte, Juan A. Fundación Instituto Leloir; Argentina.
dc.descriptionFil: Mondotte, Juan A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.descriptionFil: Byk, Laura A. Fundación Instituto Leloir; Argentina.
dc.descriptionFil: Byk, Laura A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.descriptionFil: de Maio, Federico A. Fundación Instituto Leloir; Argentina.
dc.descriptionFil: de Maio, Federico A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.descriptionFil: Samsa, Marcelo M. Fundación Instituto Leloir; Argentina.
dc.descriptionFil: Samsa, Marcelo M. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.descriptionFil: Alvarez, Cecilia I. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba; Argentina
dc.descriptionFil: Alvarez, Cecilia I. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
dc.descriptionFil: Gamarnik, Andrea V. Fundación Instituto Leloir; Argentina.
dc.descriptionFil: Gamarnik, Andrea V. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.descriptionsi
dc.descriptionDengue viruses cause the most important human viral disease transmittedby mosquitoes. In recent years, a great deal has been learnedabout molecular details of dengue virus genome replication; however,little is known about genome encapsidation and the functions of theviral capsid protein. During infection, dengue virus capsid progressivelyaccumulates around lipid droplets (LDs) by an unknown mechanism.Here, we examined the process by which the viral capsid is transportedfrom the endoplasmic reticulum (ER) membrane, where the protein issynthesized, to LDs. Using different methods of intervention, we foundthat the GBF1-Arf1/Arf4-COPI pathway is necessary for capsid transportto LDs, while the process is independent of both COPII componentsand Golgi integrity. The transport was sensitive to Brefeldin A, while adrug resistant form of GBF1 was sufficient to restore capsid subcellulardistribution in infected cells. The mechanism by which LDs gain or loseproteins is still an open question. Our results support a model in whichthe virus uses a non-canonical function of the COPI system for capsidaccumulation on LDs, providing new ideas for antiviral strategies.
dc.formatapplication/pdf
dc.languageen
dc.relation16
dc.relationTraffic
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/4.0/
dc.subjectCiencias Biológicas
dc.subjectDengue Virus
dc.subjectCapsid Protein
dc.subjectCOPI
dc.subjectGBF1
dc.subjectFlavivirus
dc.subjectLipid Droplets
dc.subjectCiencias Biológicas
dc.titleDengue Virus Uses a Non-Canonical Function of the Host GBF1-Arf-COPI System for Capsid Protein Accumulation on Lipid Droplets


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