Chicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisa

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorOliveira, GH
dc.creatorBerchieri Jr, A
dc.creatorMontassier, Helio Jose
dc.date2014-05-20T13:15:55Z
dc.date2014-05-20T13:15:55Z
dc.date2006-03-01
dc.date.accessioned2017-04-05T19:32:57Z
dc.date.available2017-04-05T19:32:57Z
dc.identifierRevista Brasileira de Ciência Avícola. Fundação APINCO de Ciência e Tecnologia Avícolas, v. 8, n. 1, p. 51-54, 2006.
dc.identifier1516-635X
dc.identifierhttp://hdl.handle.net/11449/2927
dc.identifier10.1590/S1516-635X2006000100008
dc.identifierS1516-635X2006000100008
dc.identifierS1516-635X2006000100008.pdf
dc.identifierhttp://dx.doi.org/10.1590/S1516-635X2006000100008
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/852733
dc.descriptionThis study evaluated two enzyme-linked immunosorbent assays (ELISA) in the detection of chicken serologic response against Salmonella enterica sorotype Typhimurium. The assays have used as detecting antigen the soluble bacterial proteins of a non-flagellated strain of Salmonella Typhimurium (AgTM), and antibody conjugated to peroxidase or alkaline phosphatase. According to the results, optimal dilutions of antigen (concentration 5.49 mg/mL) and serum samples in both assays were 1:20,000 and 1:1,000, respectively. In such conditions, the ELISA/AgTM was able to detect serological response to Salmonella Typhimurium. Cross-reactions to Salmonella serotypes Gallinarum and Pullorum were seen, but not with other serotypes such as Enteritidis.
dc.languageeng
dc.publisherFundação APINCO de Ciência e Tecnologia Avícolas
dc.relationRevista Brasileira de Ciência Avícola
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectChicken
dc.subjectELISA
dc.subjectenzyme
dc.subjectSalmonella sp
dc.subjectSerology
dc.titleChicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisa
dc.typeOtro


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