The effects of ovalbumin as a protein source during the in vitro production of bovine embryos

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorTetzner, Tatiane Almeida Drummond
dc.creatorSaraiva, Naiara Zoccal
dc.creatorPerecin, Felipe
dc.creatorNiciura, Simone Cristina Méo
dc.creatorFerreira, Christina Ramires
dc.creatorOliveira, Clara Slade
dc.creatorGarcia, Joaquim Mansano
dc.date2014-05-20T13:15:28Z
dc.date2014-05-20T13:15:28Z
dc.date2011-10-01
dc.date.accessioned2017-04-05T19:30:59Z
dc.date.available2017-04-05T19:30:59Z
dc.identifierRevista Brasileira de Zootecnia. Sociedade Brasileira de Zootecnia, v. 40, n. 10, p. 2135-2141, 2011.
dc.identifier1516-3598
dc.identifierhttp://hdl.handle.net/11449/2599
dc.identifier10.1590/S1516-35982011001000010
dc.identifierS1516-35982011001000010
dc.identifierWOS:000297341400010
dc.identifierS1516-35982011001000010.pdf
dc.identifierhttp://dx.doi.org/10.1590/S1516-35982011001000010
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/852488
dc.descriptionEmbryo quality is influenced by the culture conditions that affect in vitro maturation (IVM), fertilization (IVF) and culture (IVC) rates. The present study investigated the feasibility of producing bovine embryos after the replacement of fetal calf serum (FCS) and bovine serum albumin (BSA) by ovalbumin (OVA). The IVM and IVC medium were supplemented with 10% FCS, 4 mg/mL BSA, or 4 mg/mL OVA. The IVF medium was supplemented with 6 mg/mL BSA or OVA. For IVM, supplementation with FCS, BSA, and OVA did not affect nuclear maturation or cortical granule migration. Higher rates of formation of two pronuclei were obtained when FCS was employed for IVM (79.97%), regardless of the supplement used for IVF, and when BSA was used for IVF (59.4%), regardless of the supplement used for IVM. Supplementation with OVA for IVM+IVC (20.40%) and for IVF (22.15%) was inferior to supplementation with FCS for IVM+IVC (30.47%) and with BSA for IVF (28.91%) for blastocyst development. Hatching rates were lower using OVA for IVM+IVC (23.02%) and for IVF (28.93%) compared with FCS and BSA under the same conditions (40.78 and 34.82%, respectively) and BSA for IVF (36.82%). Supplementation with OVA for IVM+IVC and IVF resulted in reduced inner cell mass, trophectoderm cells and total blastocyst cell numbers (17.29, 37.88, and 55.17, respectively). In conclusion, OVA is a protein source for bovine in vitro embryo production, although the quantity and quality of bovine blastocysts using only ovalbumin in the entire in vitro production process are lower than those obtained in the presence of FCS and BSA, when used as supplements in any step of bovine in vitro embryo production.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageeng
dc.publisherSociedade Brasileira de Zootecnia
dc.relationRevista Brasileira de Zootecnia
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectbovine embryo
dc.subjectin vitro culture
dc.subjectOvalbumin
dc.subjectProtein source
dc.titleThe effects of ovalbumin as a protein source during the in vitro production of bovine embryos
dc.typeOtro


Este ítem pertenece a la siguiente institución