Infection by Brazilian and Dutch swine hepatitis E virus strains induces haematological changes in Macaca fascicularis

dc.creatorde Carvalho, Lilian G
dc.creatorMarchevsky, Renato S
dc.creatordos Santos, Debora R L
dc.creatorde Oliveira, Jaqueline M
dc.creatorde Paula, Vanessa S
dc.creatorLopes, Leilane M
dc.creatorVan der Poel, Wilhelmus H M
dc.creatorGonzález, Jorge E.
dc.creatorMunné, María Silvina
dc.creatorMoran, Julio
dc.creatorCajaraville, Ana Carolina R A
dc.creatorPelajo-Machado, Marcelo
dc.creatorCruz, Oswaldo G
dc.creatorPinto, M A
dc.date2021-02-01T20:22:25Z
dc.date2021-02-01T20:22:25Z
dc.date2013-10-23
dc.date.accessioned2023-08-29T20:09:20Z
dc.date.available2023-08-29T20:09:20Z
dc.identifier1471-2334
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/2294
dc.identifier10.1186/1471-2334-13-495
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8520626
dc.descriptionFil: de Carvalho, Lilian G. Oswaldo Cruz Foundation.Department of Primatology. Centre for Laboratory Animal Breeding, Rio de Janeiro; Brasil.
dc.descriptionFil: Marchevsky, Renato S. Oswaldo Cruz Foundation. Bio-Manguinhos. Institute of Technology on Immunobiologicals. Laboratory of Neurovirulence, Rio de Janeiro; Brasil.
dc.descriptionFil: dos Santos, Debora R. L. UFRRJ. Department of Veterinary Microbiology and Immunology. Laboratory of Veterinary Viruses, Rio de Janeiro; Brasil.
dc.descriptionFil: de Oliveira, Jaqueline M. Oswaldo Cruz Institute/Oswaldo Cruz Foundation. Laboratory of Technological Development in Virology, Rio de Janeiro; Brasil.
dc.descriptionFil: de Paula, Vanessa S. Oswaldo Cruz Institute/Oswaldo Cruz Foundation. Laboratory of Technological Development in Virology, Rio de Janeiro; Brasil.
dc.descriptionFil: Lopes, Leilane M. Oswaldo Cruz Institute/Oswaldo Cruz Foundation. Laboratory of Technological Development in Virology, Rio de Janeiro; Brasil.
dc.descriptionFil: Van der Poel, Wilhelmus H. M. Institute of Wageningen University and Research Centre. Central Veterinary; Países Bajos.
dc.descriptionFil: González, Jorge E. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: Munné, Maria S. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: Moran, Julio. Dr. Julio Moran Laboratories; Suiza.
dc.descriptionFil: Cajaraville, Ana Carolina R. A. Oswaldo Cruz Foundation. Bio-Manguinhos. Institute of Technology on Immunobiologicals. Laboratory of Virological Technology, Rio de Janeiro; Brasil.
dc.descriptionFil: Pelajo-Machado, Marcelo. Oswaldo Cruz Institute/Oswaldo Cruz Foundation. Laboratory of Pathology, Rio de Janeiro; Brasil.
dc.descriptionFil: Cruz, Oswaldo G. Oswaldo Cruz Foundation. Programme of Scientific Computation, Rio de Janeiro; Brasil.
dc.descriptionFil: Pinto, Marcelo A. Oswaldo Cruz Institute/Oswaldo Cruz Foundation. Laboratory of Technological Development in Virology, Rio de Janeiro; Brasil.
dc.descriptionBackground Hepatitis E virus (HEV) has been described as an emerging pathogen in Brazil and seems to be widely disseminated among swine herds. An autochthonous human case of acute hepatitis E was recently reported. To obtain a better understanding of the phenotypic profiles of both human and swine HEV strains, a experimental study was conducted using the animal model, Macaca fascicularis. Methods Six cynomolgus monkeys (Macaca fascicularis) were inoculated intravenously with swine HEV genotype 3 that was isolated from naturally and experimentally infected pigs in Brazil and the Netherlands. Two other monkeys were inoculated with HEV genotype 3 that was recovered from Brazilian and Argentinean patients with locally acquired acute and fulminant hepatitis E. The haematological, biochemical, and virological parameters of all animals were monitored for 67 days. Results Subclinical hepatitis was observed in all monkeys after inoculation with HEV genotype 3 that was recovered from the infected swine and human patients. HEV RNA was detected in the serum and/or faeces of 6 out of the 8 cynomolgus monkeys between 5 and 53 days after inoculation. The mild inflammation of liver tissues and elevations of discrete liver enzymes were observed. Seroconversions to anti-HEV IgM and/or IgG were detected in 7 animals. Reactivities to anti-HEV IgA were also detected in the salivary samples of 3 animals. Interestingly, all of the infected monkeys showed severe lymphopenia and a trend toward monocytosis, which coincided with elevations in alanine aminotransferase and antibody titres. Conclusions The ability of HEV to cross the species barrier was confirmed for both the swine (Brazilian and Dutch) and human (Argentinean) strains, thus reinforcing the zoonotic risk of hepatitis E in South America. Cynomolgus monkeys that were infected with HEV genotype 3 developed subclinical hepatitis that was associated with haematological changes. Haematological approaches should be considered in future studies of HEV infection.
dc.languageen
dc.relationBMC infectious diseases
dc.rightsopen
dc.subjectEnterovirus Humano B
dc.subjectLinfopenia
dc.subjectFallo Hepático Agudo
dc.subjectMacaca fascicularis
dc.titleInfection by Brazilian and Dutch swine hepatitis E virus strains induces haematological changes in Macaca fascicularis
dc.typeArtículo


Este ítem pertenece a la siguiente institución