New patterns of methicillin-resistant Staphylococcus aureus (MRSA) clones, community-associated MRSA genotypes behave like healthcare-associated MRSA genotypes within hospitals, Argentina

dc.creatorEgea, Ana L
dc.creatorGagetti, Paula
dc.creatorLamberghini, Ricardo
dc.creatorFaccone, Diego
dc.creatorLucero, Celeste
dc.creatorVindel, Ana
dc.creatorTosoroni, Dario
dc.creatorGarnero, Analía
dc.creatorSaka, Héctor A.
dc.creatorGalas, Marcelo F.
dc.creatorBocco, Jose Luis
dc.creatorCorso, Alejandra
dc.creatorSola, Claudia
dc.creatorS. aureus Study Group Argentina
dc.date2021-02-01T20:10:24Z
dc.date2021-02-01T20:10:24Z
dc.date2014-11
dc.date.accessioned2023-08-29T20:09:16Z
dc.date.available2023-08-29T20:09:16Z
dc.identifier1618-0607
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/2275
dc.identifier10.1016/j.ijmm.2014.08.002
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8520590
dc.descriptionFil: Egea, Ana L. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Centro de Investigaciones en Bioquímica Clínica e Inmunología. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina.
dc.descriptionFil: Gagetti, Paula. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
dc.descriptionFil: Lamberghini, Ricardo. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra de Infectología I. Hospital Rawson; Argentina.
dc.descriptionFil: Faccone, Diego. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
dc.descriptionFil: Lucero, Celeste. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
dc.descriptionFil: Vindel, Ana. Centro Nacional de Microbiología. Instituto de Salud Carlos III. Laboratorio de Infecciones Nosocomiales; España.
dc.descriptionFil: Tosoroni, Dario. Universidad Católica de Córdoba. Facultad de Medicina. Área de Informática Médica; Argentina.
dc.descriptionFil: Garnero, Analía. Hospital de Niños de la Santísima Trinidad. Servicio de Infectología, Córdoba; Argentina.
dc.descriptionFil: Saka, Hector A. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Centro de Investigaciones en Bioquímica Clínica e Inmunología. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina.
dc.descriptionFil: Galas, Marcelo. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
dc.descriptionFil: Bocco, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Centro de Investigaciones en Bioquímica Clínica e Inmunología. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina.
dc.descriptionFil: Corso, Alejandra. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
dc.descriptionFil: Sola, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Centro de Investigaciones en Bioquímica Clínica e Inmunología. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina.
dc.descriptionFil: S. aureus Study Group; Argentina.
dc.descriptionMethicillin-resistant Staphylococcus aureus (MRSA) burden is increasing worldwide in hospitals [healthcare-associated (HA)-MRSA] and in communities [community-associated (CA)-MRSA]. However, the impact of CA-MRSA within hospitals remains limited, particularly in Latin America. A countrywide representative survey of S. aureus infections was performed in Argentina by analyzing 591 clinical isolates from 66 hospitals in a prospective cross-sectional, multicenter study (Nov-2009). This work involved healthcare-onset infections-(HAHO, >48 hospitalization hours) and community-onset (CO) infections [including both, infections (HACO) in patients with healthcare-associated risk-factors (HRFs) and infections (CACO) in those without HRFs]. MRSA strains were genetically typed as CA-MRSA and HA-MRSA genotypes (CA-MRSAG and HA-MRSAG) by SCCmec- and spa-typing, PFGE, MLST and virulence genes profile by PCR. Considering all isolates, 63% were from CO-infections and 55% were MRSA [39% CA-MRSAG and 16% HA-MRSAG]. A significantly higher MRSA proportion among CO- than HAHO-S. aureus infections was detected (58% vs 49%); mainly in children (62% vs 43%). The CA-MRSAG/HA-MRSAG have accounted for 16%/33% of HAHO-, 39%/13% of HACO- and 60.5%/0% of CACO-infections. Regarding the epidemiological associations identified in multivariate models for patients with healthcare-onset CA-MRSAG infections, CA-MRSAG behave like HA-MRSAG within hospitals but children were the highest risk group for healthcare-onset CA-MRSAG infections. Most CA-MRSAG belonged to two major clones: PFGE-type N-ST30-SCCmecIVc-t019-PVL(+) and PFGE-type I-ST5-IV-SCCmecIVa-t311-PVL(+) (45% each). The ST5-IV-PVL(+)/ST30-IV-PVL(+) clones have caused 31%/33% of all infections, 20%/4% of HAHO-, 43%/23% of HACO- and 35%/60% of CACO- infections, with significant differences by age groups (children/adults) and geographical regions. Importantly, an isolate belonging to USA300-0114-(ST8-SCCmecIVa-spat008-PVL(+)-ACME(+)) was detected for the first time in Argentina. Most of HA-MRSAG (66%) were related to the Cordobes/Chilean clone-(PFGE-type A-ST5-SCCmecI-t149) causing 18% of all infections (47% of HAHO- and 13% of HACO-infections). Results strongly suggest that the CA-MRSA clone ST5-IV-PVL(+) has begun to spread within hospitals, replacing the traditional Cordobes/Chilean-HA-MRSA clone ST5-I-PVL(-), mainly in children. Importantly, a growing MRSA reservoir in the community was associated with spreading of two CA-MRSA clones: ST5-IV-PVL(+), mainly in children with HRFs, and ST30-IV-PVL(+) in adults without HRFs. This is the first nationwide study in Argentina providing information about the molecular and clinical epidemiology of CA-MRSA, particularly within hospitals, which is essential for designing effective control measures in this country and worldwide.
dc.languageen
dc.relationInternational journal of medical microbiology : IJMM
dc.rightsnone
dc.subjectArgentina
dc.subjectStaphylococcus aureus Resistente a Meticilina
dc.subjectStaphylococcus aureus
dc.titleNew patterns of methicillin-resistant Staphylococcus aureus (MRSA) clones, community-associated MRSA genotypes behave like healthcare-associated MRSA genotypes within hospitals, Argentina
dc.typeArtículo


Este ítem pertenece a la siguiente institución