Performance of a PCR assay for the rapid identification of the Klebsiella pneumoniae ST258 epidemic clone in Latin American clinical isolates

dc.creatorGomez, Sonia A.
dc.creatorRapoport, Melina J.
dc.creatorPiergrossi, N
dc.creatorFaccone, Diego
dc.creatorPasteran, Fernando
dc.creatorDe Belder, Denise
dc.creatorReLAVRA-Group
dc.creatorPetroni, Alejandro
dc.creatorCorso, Alejandra
dc.date2021-01-20T12:54:02Z
dc.date2021-01-20T12:54:02Z
dc.date2016-10
dc.date.accessioned2023-08-29T20:09:00Z
dc.date.available2023-08-29T20:09:00Z
dc.identifier1567-1348
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/2182
dc.identifier10.1016/j.meegid.2016.06.018
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8520451
dc.descriptionFil: Gómez, Sonia Alejandra. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
dc.descriptionFil: Rapoport, Melina J. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
dc.descriptionFil: Piergrossi, N. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
dc.descriptionFil: Faccone, Diego. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
dc.descriptionFil: Pasteran, Fernando. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
dc.descriptionFil: De Belder, Denise. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
dc.descriptionFil: ReLAVRA-Group. Red Latinoamericana de Vigilancia de la Resistencia a los Antimicrobianos (ReLAVRA).
dc.descriptionFil: Petroni, Alejandro. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
dc.descriptionFil: Corso, Alejandra. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
dc.descriptionThe worldwide dissemination of Klebsiella pneumoniae carbapenemase (KPC)-producing Klebsiella pneumoniae ST258 demands a rapid PCR-based typing method to detect unique genes of the ST258 clone. This study evaluates a PCR developed by Adler et al. (2014) for the detection of ST258 in K. pneumoniae clinical isolates centered on the identification of the pilv-I and prp genes. We tested 143 clinical isolates from Argentina (n=109), Chile (n=1), Colombia (n=1), Costa Rica (n=2), Ecuador (n=5), El Salvador (n=2), Nicaragua (n=5), Panamá (n=2), Paraguay (n=2), Perú (n=3) and Trinidad and Tobago (n=11) recovered from 2006 to 2015. blaKPC, pilv-l and prp genes were detected by PCR and sequenced by standard procedures. ST258 and non-ST258 were defined by PFGE and/or MLST. Isolates were grouped according to PFGE patterns: 58 were compatible with ST258 (group 1) and 85 with non-ST258 (group 2). MLST study was done on an arbitrary selection of isolates. The pilv-l gene was present only in ST258 isolates, regardless of the presence of the blaKPC gene. Results for the prp gene were variable. Its presence did not define ST258. The pilv-I PCR had a sensitivity and specificity of 100%, respectively, for the detection of ST258 in the isolates under investigation. Given our findings, the pilv-I PCR could replace more time and resource consuming methods, allowing for more rapid detection of the circulating high risk K. pneumoniae clone ST258 in Latin American (LA) countries.
dc.formatpdf
dc.languageen
dc.relationInfection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
dc.rightsnone
dc.sourceInfection, Genetics and Evolution 2016;44:145-146
dc.subjectKlebsiella pneumoniae
dc.subjectReacción en Cadena de la Polimerasa
dc.subjectAmérica Latina
dc.titlePerformance of a PCR assay for the rapid identification of the Klebsiella pneumoniae ST258 epidemic clone in Latin American clinical isolates
dc.typeArtículo


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