Phylogenetically related Argentinean and Australian Escherichia coli O157 isolates are distinguished by virulence clades and alternative Shiga toxin 1 and 2 prophages

dc.creatorMellor, Glen E
dc.creatorSim, Eby M
dc.creatorBarlow, Robert S
dc.creatorD'Astek, Beatriz A
dc.creatorGalli, Lucía
dc.creatorChinen, Isabel
dc.creatorRivas, Marta
dc.creatorGobius, Kari
dc.date2021-01-20T13:23:07Z
dc.date2021-01-20T13:23:07Z
dc.date2012-07
dc.date.accessioned2023-08-29T20:08:56Z
dc.date.available2023-08-29T20:08:56Z
dc.identifier1098-5336
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/2196
dc.identifier10.1128/AEM.00365-12
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8520420
dc.descriptionFil: Mellor, Glen E. CSIRO Food and Nutritional Sciences, Archerfield Business Centre, Queensland; Australia.
dc.descriptionFil: Sim, Eby M. CSIRO Food and Nutritional Sciences, Archerfield Business Centre, Queensland; Australia.
dc.descriptionFil: Barlow, Robert S. CSIRO Food and Nutritional Sciences, Archerfield Business Centre, Queensland; Australia.
dc.descriptionFil: D'Astek, Beatriz A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
dc.descriptionFil: Galli, Lucia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
dc.descriptionFil: Chinen, Isabel. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
dc.descriptionFil: Rivas, Marta. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
dc.descriptionFil: Gobius, Kari S. CSIRO Food and Nutritional Sciences, Archerfield Business Centre, Queensland; Australia.
dc.descriptionShiga toxigenic Escherichia coli O157 is the leading cause of hemolytic uremic syndrome (HUS) worldwide. The frequencies of stx genotypes and the incidences of O157-related illness and HUS vary significantly between Argentina and Australia. Locus-specific polymorphism analysis revealed that lineage I/II (LI/II) E. coli O157 isolates were most prevalent in Argentina (90%) and Australia (88%). Argentinean LI/II isolates were shown to belong to clades 4 (28%) and 8 (72%), while Australian LI/II isolates were identified as clades 6 (15%), 7 (83%), and 8 (2%). Clade 8 was significantly associated with Shiga toxin bacteriophage insertion (SBI) type stx(2) (locus of insertion, argW) in Argentinean isolates (P < 0.0001). In Argentinean LI/II strains, stx(2) is carried by a prophage inserted at argW, whereas in Australian LI/II strains the argW locus is occupied by the novel stx(1) prophage. In both Argentinean and Australian LI/II strains, stx(2c) is almost exclusively carried by a prophage inserted at sbcB. However, alternative q(933)- or q(21)-related alleles were identified in the Australian stx(2c) prophage. Argentinean LI/II isolates were also distinguished from Australian isolates by the presence of the putative virulence determinant ECSP_3286 and the predominance of motile O157:H7 strains. Characteristics common to both Argentinean and Australian LI/II O157 strains included the presence of putative virulence determinants (ECSP_3620, ECSP_0242, ECSP_2687, ECSP_2870, and ECSP_2872) and the predominance of the tir255T allele. These data support further understanding of O157 phylogeny and may foster greater insight into the differential virulence of O157 lineages.
dc.formatpdf
dc.languageen
dc.relationApplied and Environmental Microbiology
dc.rightsnone
dc.sourceApplied and Environmental Microbiology 2012;78(13):4724–4731
dc.subjectEscherichia coli O157
dc.subjectToxina Shiga
dc.subjectArgentina
dc.subjectAustralia
dc.subjectEscherichia coli Shiga-Toxigénica
dc.titlePhylogenetically related Argentinean and Australian Escherichia coli O157 isolates are distinguished by virulence clades and alternative Shiga toxin 1 and 2 prophages
dc.typeArtículo


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