UV-MALDI mass spectrometry analysis of NBD-glycosphingolipids without an external matrix

dc.creatorLandoni, Malena
dc.creatorDuschak, Vilma G
dc.creatorErra-Balsells, Rosa
dc.creatorCouto, Alicia S
dc.date2021-01-10T23:49:26Z
dc.date2021-01-10T23:49:26Z
dc.date2008-07
dc.date.accessioned2023-08-29T20:08:36Z
dc.date.available2023-08-29T20:08:36Z
dc.identifier1044-0305
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/2082
dc.identifier10.1016/j.jasms.2008.04.017
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8520246
dc.descriptionFil: Landoni, Malena. CIHIDECAR-Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires; Argentina.
dc.descriptionFil: Duschak, Vilma G. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.
dc.descriptionFil: Erra-Balsells, Rosa. Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria; Argentina.
dc.descriptionFil: Couto, Alicia S. Departamento de Quimica Organica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires; Argentina.
dc.descriptionEach day, advances in the instrumentation and operating protocols bring new applications and insights into the molecular processes of ultra violet-matrix assisted laser desorption/ionization-mass spectrometry (UV-MALDI MS), increasing its potential use. We report here an approach in which mass spectrometry analysis of sphingolipids has been performed using a fluorescent tag (nitrobenz-2-oxa-1, 3-diazole, NBD) covalently linked to the sphingoid base as matrix. Thus, different labeled-sphingolipids were analyzed: ceramide, dihydroceramide, acetylceramide, glucosylceramide, galactosylceramide, galactosyldihydroceramide. In addition an extract of glycosphingolipids obtained from epimastigote forms of Trypanosoma cruzi metabolically labeled with NBD-ceramide was analyzed. The goal of this work is to show that no matrix needs to be added for the mass spectrometry analysis as the same tag used to label the lipids may generate efficiently analyte ions to obtain high quality signals.
dc.languageen
dc.publisherAmerican Chemical Society
dc.relationJournal of the American Society for Mass Spectrometry
dc.rightsnone
dc.sourceJournal of the American Society for Mass Spectrometry 2008; 19(7):923-6.
dc.subjectAnálisis Espectral
dc.subjectGlicoesfingolípidos
dc.titleUV-MALDI mass spectrometry analysis of NBD-glycosphingolipids without an external matrix
dc.typeArtículo


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