First detection of Cryptosporidium DNA in blood and cerebrospinal fluid of HIV-infected patients

dc.creatorVelásquez, Jorge Néstor
dc.creatorPantano, María Laura
dc.creatorVittar, Natalia
dc.creatorNigro, Mónica Gabriela
dc.creatorFigueiras, Olga
dc.creatorAstudillo, Osvaldo Germán
dc.creatorRicart, Javier
dc.creatorDella Paolera, Daniela
dc.creatorCarnevale, Silvana
dc.date2020-12-18T16:00:53Z
dc.date2020-12-18T16:00:53Z
dc.date2018-03
dc.date.accessioned2023-08-29T20:08:01Z
dc.date.available2023-08-29T20:08:01Z
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1944
dc.identifier10.1007/s00436-018-5766-1
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519955
dc.descriptionFil: Velásquez, Jorge Néstor. Hospital Municipal de Infecciosas Dr. Francisco Javier Muñiz; Argentina.
dc.descriptionFil: Pantano, María Laura. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.
dc.descriptionFil: Vittar, Natalia. Hospital Municipal de Infecciosas Dr. Francisco Javier Muñiz; Argentina.
dc.descriptionFil: Nigro, Mónica Gabriela. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.
dc.descriptionFil: Figueiras, Olga. Hospital Municipal de Infecciosas Dr. Francisco Javier Muñiz; Argentina.
dc.descriptionFil: Astudillo, Osvaldo Germán. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.
dc.descriptionFil: Ricart, Javier. Hospital Municipal de Infecciosas Dr. Francisco Javier Muñiz; Argentina.
dc.descriptionFil: Della Paolera, Daniela. Hospital Municipal de Infecciosas Dr. Francisco Javier Muñiz; Argentina.
dc.descriptionFil: Carnevale, Silvana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.
dc.descriptionHuman cryptosporidiosis is an intestinal infection caused by different species belonging to the genus Cryptosporidium in both immunocompetent and immunocompromised individuals. The life cycle of Cryptosporidium sp. when affecting the digestive system is well known but the infection of other organs is less studied. Molecular methods are necessary for species and subtypes identification. The goal of this work is to propose a new approach that contributes to the diagnosis of the extra-intestinal dissemination process of Cryptosporidium infection. Cryptosporidium sp. was detected in stool and biopsy samples of two HIV-infected patients. DNA was extracted from feces, biopsy specimens, blood, and cerebrospinal fluid (CSF). All samples were analyzed by nested PCR-RFLP of the 18S rDNA, real-time PCR, and gp60 subtyping. Cryptosporidium DNA was detected in stool and tissue samples and it was also present in blood and CSF samples. Both cases were characterized as Cryptosporidium hominis subtype IeA11G3T3. This is the first report that demonstrates the presence of Cryptosporidium DNA in blood and CSF of HIV-infected patients.
dc.languageen
dc.publisherSpringer
dc.relation#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.relationdatasets
dc.relationParasitology research
dc.rightsnone
dc.sourceParasitology Research 2018; 117(3):875-881
dc.subjectSíndrome de Inmunodeficiencia Adquirida
dc.subjectSangre
dc.subjectFactores Estimulantes de Colonias
dc.subjectReacción en Cadena de la Polimerasa
dc.subjectAdulto
dc.subjectAnimales
dc.subjectCryptosporidiosis
dc.subjectCryptosporidium
dc.subjectADN Protozoario
dc.subjectADN Ribosómico
dc.subjectHeces
dc.subjectInfecciones por VIH
dc.subjectHumanos
dc.subjectHuésped Inmunocomprometido
dc.subjectMasculino
dc.subjectPersona de Mediana Edad
dc.subjectPolimorfismo de Longitud del Fragmento de Restricción
dc.subjectReacción en Cadena en Tiempo Real de la Polimerasa
dc.titleFirst detection of Cryptosporidium DNA in blood and cerebrospinal fluid of HIV-infected patients
dc.typeArtículo


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