APC senses cell-cell contacts and moves to the nucleus upon their disruption

dc.creatorBrocardo, Mariana G.
dc.creatorBianchini, Michele
dc.creatorRadrizzani, Martin
dc.creatorReyes, Gloria B.
dc.creatorDugour, Andrea V.
dc.creatorTaminelli, Guillermo L.
dc.creatorGonzalez Solveyra, César
dc.creatorSanta-Coloma, Tomás A.
dc.date2020-12-18T15:58:49Z
dc.date2020-12-18T15:58:49Z
dc.date2001-06-22
dc.date.accessioned2023-08-29T20:07:59Z
dc.date.available2023-08-29T20:07:59Z
dc.identifier0006-291X
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1937
dc.identifier10.1006/bbrc.2001.5066
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519938
dc.descriptionFil: Brocardo, Mariana G. Instituto de Investigaciones Bioquímicas. Fundación Campomar; Argentina.
dc.descriptionFil: Bianchini, Michele. Instituto de Investigaciones Bioquímicas. Fundación Campomar; Argentina.
dc.descriptionFil: Radrizzani, Martín. ANLIS Dr.C.G.Malbrán. Centro Nacional de Genética Médica; Argentina.
dc.descriptionFil: Reyes, Gloria B. Instituto de Investigaciones Bioquímicas. Fundación Campomar; Argentina.
dc.descriptionFil: Dugour, Andrea V. Instituto de Investigaciones Bioquímicas. Fundación Campomar; Argentina.
dc.descriptionFil: Taminelli, Guillermo L. Instituto de Investigaciones Bioquímicas. Fundación Campomar; Argentina.
dc.descriptionFil: Gonzalez Solveyra, César. Instituto de Investigaciones Bioquímicas. Fundación Campomar; Argentina.
dc.descriptionFil: Santa-Coloma, Tomás A. Instituto de Investigaciones Bioquímicas. Fundación Campomar; Argentina.
dc.descriptionThe adenomatous polyposis coli (APC) tumor suppressor protein is involved in the Wnt/wingless pathway, modulating beta-catenin activity. We report the development of a highly specific, chemically synthesized oligobody (oligonucleotide-based synthetic antibody), directed against the N-terminal region of APC. Using this reagent, we found that within 16 h of disrupting HT-29 cell-cell contacts by harvesting cells with trypsin/EDTA treatment and replating, APC was translocated from the cytoplasm to the nucleus. Five days after plating the cells, when the cells had returned to their normal confluent phenotype and cell-cell contacts were reestablished, APC returned to the cytoplasm. These results suggest that APC functions as part of a "sensor" system, and responds to the loss of cell-cell contacts by moving to the nucleus, and returning to the cytoplasm when the contacts are fully restored.
dc.languageen
dc.relationBiochemical and biophysical research communications
dc.rightsnone
dc.subjectProteína de la Poliposis Adenomatosa del Colon
dc.subjectNeoplasias del Colon
dc.subjectProteínas del Citoesqueleto
dc.subjectGenes APC
dc.subjectUniones Intercelulares
dc.subjectCélulas Tumorales Cultivadas
dc.titleAPC senses cell-cell contacts and moves to the nucleus upon their disruption
dc.typeArtículo


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