Antifungal susceptibility testing of Malassezia yeast: comparison of two different methodologies

dc.creatorRojas, Florencia D
dc.creatorCórdoba, Susana
dc.creatorSosa, María de los A
dc.creatorZalazar, Laura C
dc.creatorFernandez, Mariana
dc.creatorCattana, María E
dc.creatorAlegre, Liliana R
dc.creatorCarrillo-Muñoz, Alfonso
dc.creatorGiusiano, Gustavo E
dc.date2020-12-16T10:08:42Z
dc.date2020-12-16T10:08:42Z
dc.date2017-02
dc.date.accessioned2023-08-29T20:07:53Z
dc.date.available2023-08-29T20:07:53Z
dc.identifier1439-0507
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1914
dc.identifier10.1111/myc.12556
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519891
dc.descriptionFil: Rojas, Florencia D. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.
dc.descriptionFil: Córdoba, Susana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.
dc.descriptionFil: Sosa, María de los A. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.
dc.descriptionFil: Zalazar, Laura C. Universidad Nacional del Nordeste. Facultad de Humanidades. Cátedra de Matématica Aplicada; Chaco, Argentina.
dc.descriptionFil: Fernández, Mariana S. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.
dc.descriptionFil: Cattana, María E. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.
dc.descriptionFil: Alegre, Liliana R. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.
dc.descriptionFil: Carrillo-Muñoz, Alfonso. ACIAM. Departamento de Microbiología; Barcelona, España.
dc.descriptionFil: Giusiano, Gustavo E. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.
dc.descriptionAll Malassezia species are lipophilic; thus, modifications are required in susceptibility testing methods to ensure their growth. Antifungal susceptibility of Malassezia species using agar and broth dilution methods has been studied. Currently, few tests using disc diffusion methods are being performed. The aim was to evaluate the in vitro susceptibility of Malassezia yeast against antifungal agents using broth microdilution and disc diffusion methods, then to compare both methodologies. Fifty Malassezia isolates were studied. Microdilution method was performed as described in reference document and agar diffusion test was performed using antifungal tablets and discs. To support growth, culture media were supplemented. To correlate methods, linear regression analysis and categorical agreement was determined. The strongest linear association was observed for fluconazole and miconazole. The highest agreement between both methods was observed for itraconazole and voriconazole and the lowest for amphotericin B and fluconazole. Although modifications made to disc diffusion method allowed to obtain susceptibility data for Malassezia yeast, variables cannot be associated through a linear correlation model, indicating that inhibition zone values cannot predict MIC value. According to the results, disc diffusion assay may not represent an alternative to determine antifungal susceptibility of Malassezia yeast.
dc.languageen
dc.relationMycoses
dc.rightsnone
dc.subjectMalassezia
dc.subjectAntifúngicos
dc.subjectAnfotericina B
dc.subjectPruebas de Sensibilidad Microbiana
dc.titleAntifungal susceptibility testing of Malassezia yeast: comparison of two different methodologies
dc.typeArtículo


Este ítem pertenece a la siguiente institución