Antifungal susceptibility of Malassezia furfur, Malassezia sympodialis, and Malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution method

dc.creatorRojas, Florencia D
dc.creatorSosa, María de los A
dc.creatorFernandez, Mariana
dc.creatorCattana, María E
dc.creatorCórdoba, Susana
dc.creatorGiusiano, Gustavo E
dc.date2020-12-16T10:08:24Z
dc.date2020-12-16T10:08:24Z
dc.date2014-08
dc.date.accessioned2023-08-29T20:07:53Z
dc.date.available2023-08-29T20:07:53Z
dc.identifier1460-2709
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1913
dc.identifier10.1093/mmy/myu010
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519889
dc.descriptionFil: Rojas, Florencia D. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.
dc.descriptionFil: Sosa, María de los A. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.
dc.descriptionFil: Fernández, Mariana S. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.
dc.descriptionFil: Cattana, María E. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.
dc.descriptionFil: Córdoba, Susana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.
dc.descriptionFil: Giusiano, Gustavo E. Universidad Nacional del Nordeste. Instituto de Medicina Regional. Departamento de Micología; Chaco, Argentina.
dc.descriptionWe studied the in vitro activity of fluconazole (FCZ), ketoconazole (KTZ), miconazole (MCZ), voriconazole (VCZ), itraconazole (ITZ) and amphotericin B (AMB) against the three major pathogenic Malassezia species, M. globosa, M. sympodialis, and M. furfur. Antifungal susceptibilities were determined using the broth microdilution method in accordance with Clinical and Laboratory Standards Institute reference document M27-A3. To support lipid-dependent yeast development, glucose, peptone, ox bile, malt extract, glycerol, and Tween supplements were added to Roswell Park Memorial Institute RPMI 1640 medium. The supplemented medium allowed good growth of all three species studied. The minimal inhibitory concentrations (MICs) were recorded after 72 h of incubation at 32ºC. The three species showed different susceptibility profiles for the drugs tested. Malassezia sympodialis was the most susceptible and M. furfur the least susceptible species. KTZ, ITZ, and VCZ were the most active drugs, showing low variability among isolates of the same species. FCZ, MCZ, and AMB showed high MICs and wide MIC ranges. Differences observed emphasize the need to accurately identify and evaluate antifungal susceptibility of Malassezia species. Further investigations and collaborative studies are essential for correlating in vitro results with clinical outcomes since the existing limited data do not allow definitive conclusions.
dc.formatPDF
dc.languageen
dc.relationMedical mycology
dc.rightsopen
dc.subjectDermatomicosis
dc.subjectMalassezia
dc.subjectAntifúngicos
dc.subjectAnfotericina B
dc.titleAntifungal susceptibility of Malassezia furfur, Malassezia sympodialis, and Malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution method
dc.typeArtículo


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