Increased frequency of rotavirus G3P[8] and G12P[8] in Argentina during 2008-2009: whole-genome characterization of emerging G12P[8] strains

dc.creatorStupka, Juan A.
dc.creatorDegiuseppe, Juan Ignacio
dc.creatorIrala Parra, Juan Gabriel
dc.creatorArgentinean National Rotavirus Surveillance Network
dc.date2020-12-15T14:31:16Z
dc.date2020-12-15T14:31:16Z
dc.date2012-06
dc.date.accessioned2023-08-29T20:07:51Z
dc.date.available2023-08-29T20:07:51Z
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1905
dc.identifier10.1016/j.jcv.2012.02.011
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519870
dc.descriptionFil: Stupka, Juan. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: Degiuseppe, Juan Ignacio. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Servicio Hepatitis y Gastroenteritis; Argentina.
dc.descriptionFil: Parra, Gabriel I. Universidad Nacional de Asunción. Instituto de Investigaciones en Ciencias de la Salud. Departamento de Biología Molecular; Paraguay.
dc.descriptionFil: Argentinean National Rotavirus Surveillance Network; Argentina.
dc.descriptionBackground: Group A rotaviruses are the leading cause of non-bacterial severe diarrhea disease in infants and young children. In humans, the most common genotypes are G1-G4 and G9. Recently, G12 strains have been sporadically reported in several countries, including Argentina, Brazil and Paraguay. Objectives: To analyze rotavirus strain diversity in Argentina during 2008-2009 and to describe the whole genome-based classification of emerging G12P[8] strains detected in our country. Study design: Rotavirus positive-samples (n=544) were collected from Argentinean children during 2008-2009, as a part of the National Surveillance Network for Viral Diarrheas. Specimens were genotyped by reverse transcription-polymerase chain reaction (RT-PCR) followed by nested-multiplex PCR. Sequencing of 11 genome segments was performed in 3 randomly selected G12P[8] strains. Results: G9P[8] was the most frequent strain in 2008, but in 2009 G3P[8] and G12P[8] were the most frequent strains in different geographical regions of the country. The novel emerging G12P[8] strains presented the following combination of genes: G12-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1 (i.e. genotype1, Wa-like strains). The phylogenetic analysis of the VP7 gene of the G12P[8] strains grouped them within lineage III. Previously reported Argentinean G12P[9] strains presented genes from genotype 3 (AU-1-like strains) with a VP7 gene from lineage II. Conclusions: The emergence of G12P[8] rotaviruses was due to the introduction of a new strain, rather than to a reassortment of the G12P[9] strains previously circulating in our country. This study assesses the temporal and geographical changes in genotypes prevalence as well as the periodic emergence of unusual G genotypes.
dc.formatpdf
dc.languageen
dc.relationJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology
dc.rightsopen
dc.subjectAnálisis por Conglomerados
dc.subjectDatos de Secuencia Molecular
dc.subjectFilogeografía
dc.subjectPolimorfismo Genético
dc.subjectPrevalencia
dc.subjectARN Viral
dc.subjectReacción en Cadena de la Polimerasa de Transcriptasa Inversa
dc.subjectRotavirus
dc.subjectAnálisis de Secuencia de ADN
dc.titleIncreased frequency of rotavirus G3P[8] and G12P[8] in Argentina during 2008-2009: whole-genome characterization of emerging G12P[8] strains
dc.typeArtículo


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