Antibody response to culture filtrate antigens of Mycobacterium tuberculosis during and after treatment of tuberculosis patients

dc.creatorImaz, María Susana
dc.creatorZerbini, Elsa V.
dc.date2020-12-14T19:35:21Z
dc.date2020-12-14T19:35:21Z
dc.date2000-06
dc.date.accessioned2023-08-29T20:07:50Z
dc.date.available2023-08-29T20:07:50Z
dc.identifier1027-3719
dc.identifierhttps://www.ingentaconnect.com/content/iuatld/ijtld/2000/00000004/00000006/art00012
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1876
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519867
dc.descriptionFil: Imaz, María Susana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Santa Fe, Argentina.
dc.descriptionFil: Zerbini, Elsa V. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Respiratorias; Santa Fe, Argentina.
dc.descriptionSETTING: Many authors have shown rising titres of antimycobacterial antibodies after a few months of antituberculosis treatment. This humoral response might persist for years, making the discrimination between current and old disease difficult. OBJECTIVE: Characterisation of the humoral response to culture filtrates of Mycobacterium tuberculosis before and after treatment of tuberculous patients in order to identify those antigens that could provide information about disease activity. METHODS: Anti-mycobacterial IgG response was determined during and after treatment of tuberculous patients by ELISA and immunoblot. Serum was taken from 71 active tuberculous patients (59 newly acquired and 12 relapse), 15 old tuberculous patients and 45 nontuberculous control subjects. RESULTS: By ELISA, antibody response increased after 2 months of treatment. After chemotherapy was completed, the estimated number of antibodies remained at the same level. The level of specific antibodies in patients seems to reach the same level as that of control subjects 3 years after initiation of treatment. In Western blot, although each patient serum had its own characteristic banding pattern, differences between tuberculous patients and control subjects were found in the area below 20 kDa. Serum from tuberculous patients showed high levels of antibodies at the 14 kDa region. After the beginning of treatment, the intensity of the 14 kDa region band and the percentage of positive recognition tended to decrease. Therefore, one year after initiation of treatment, only seven of 13 cases who demonstrated antimycobacterial antibodies in ELISA revealed a mild but still positive reaction at the 14 kDa region; this reactivity disappeared 2 years after initiation of chemotherapy. CONCLUSIONS: The 14 kDa region antigen seems to induce a humoral response that evolves in relation with the disease activity
dc.formatpdf
dc.languageen
dc.relation#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.relationdatasets
dc.relationThe international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease
dc.rightsopen
dc.subjectTuberculosis Pulmonar
dc.subjectMycobacterium tuberculosis
dc.subjectEnsayo de Inmunoadsorción Enzimática
dc.subjectFormación de Anticuerpos
dc.subjectElectroforesis en Gel Bidimensional
dc.titleAntibody response to culture filtrate antigens of Mycobacterium tuberculosis during and after treatment of tuberculosis patients
dc.typeArtículo


Este ítem pertenece a la siguiente institución