Evaluation of concentration efficiency of the Pseudomonas aeruginosa phage PP7 in various water matrixes by different methods

dc.creatorPoma, Hugo Ramiro
dc.creatorRajal, Verónica Beatriz
dc.creatorBlanco Fernández, María Dolores
dc.creatorBarril, Patricia Angélica
dc.creatorGiordano, Miguel Oscar
dc.creatorMasachessi, Gisela
dc.creatorMartínez, Laura Cecilia
dc.creatorIsa, María Beatriz
dc.creatorFreire, María Cecilia
dc.creatorLópez Riviello, Gabriela
dc.creatorCisterna, Daniel
dc.creatorNates, Silvia Viviana
dc.creatorMbayed, Viviana Andrea
dc.date2020-12-14T19:40:49Z
dc.date2020-12-14T19:40:49Z
dc.date2013-03
dc.date.accessioned2023-08-29T20:07:48Z
dc.date.available2023-08-29T20:07:48Z
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1893
dc.identifier10.1007/s10661-012-2731-9
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519846
dc.descriptionFil: Poma, Hugo Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones para la Industria Química; Argentina.
dc.descriptionFil: Rajal, Verónica Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones para la Industria Química; Argentina.
dc.descriptionFil: Blanco Fernández, María Dolores. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Virología; Argentina.
dc.descriptionFil: Barril, Patricia Angélica. Universidad Nacional de Córdoba. Instituto de Virología Dr. J. M. Vanella; Argentina.
dc.descriptionFil: Giordano, Miguel Oscar. Universidad Nacional de Córdoba. Instituto de Virología Dr. J. M. Vanella; Argentina.
dc.descriptionFil: Masachessi, Gisela. Universidad Nacional de Córdoba. Instituto de Virología Dr. J. M. Vanella; Argentina.
dc.descriptionFil: Martínez, Laura Cecilia. Universidad Nacional de Córdoba. Instituto de Virología Dr. J. M. Vanella; Argentina.
dc.descriptionFil: Isa, María Beatriz. Universidad Nacional de Córdoba. Instituto de Virología Dr. J. M. Vanella; Argentina.
dc.descriptionFil: Freire, María Cecilia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: López Riviello, Gabriela. Prefectura Naval Argentina. Departamento Científico Pericial; Argentina.
dc.descriptionFil: Cisterna, Daniel. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: Nates, Silvia Viviana. Universidad Nacional de Córdoba. Instituto de Virología Dr. J. M. Vanella; Argentina.
dc.descriptionFil: Mbayed, Viviana Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Virología; Argentina.
dc.descriptionEnteric viruses monitoring in surface waters requires the concentration of viruses before detection assays. The aim of this study was to evaluate different methods in terms of recovery efficiencies of bacteriophage PP7 of Pseudomonas aeruginosa, measured by real-time PCR, using it as a viral control process in water analysis. Different nucleic acid extraction methods (silica-guanidinium thiocyanate, a commercial kit (Qiagen Viral RNA Kit) and phenol-chloroform with alcohol precipitation) exhibited very low recovery efficiencies (0.08-4.18 %), being the most efficient the commercial kit used for subsequent experiments. To evaluate the efficiency of three concentration methods, PBS (as model for clean water) and water samples from rivers were seeded to reach high (HC, 10(6) pfu ml(-1)) and low concentrations (LC, 10(4) pfu ml(-1)) of PP7. Tangential ultrafiltration proved to be more efficient (50.36 ± 12.91, 17.21 ± 9.22 and 12.58 ± 2.35 % for HC in PBS and two river samples, respectively) than adsorption-elution with negatively charged membranes (1.00 ± 1.34, 2.79 ± 2.62 and 0.05 ± 0.08 % for HC in PBS and two river samples, respectively) and polyethylene glycol precipitation (15.95 ± 7.43, 4.01 ± 1.12 and 3.91 ± 0.54 %, for HC in PBS and two river samples, respectively), being 3.2-50.4 times more efficient than the others for PBS and 2.7-252 times for river samples. Efficiencies also depended on the initial virus concentration and aqueous matrixes composition. In consequence, the incorporation of an internal standard like PP7 along the process is useful as a control of the water concentration procedure, the nucleic acid extraction, the presence of inhibitors and the variability of the recovery among replicas, and for the calculation of the sample limit of detection. Thus, the use of a process control, as presented here, is crucial for the accurate quantification of viral contamination.
dc.formatpdf
dc.languageen
dc.publisherSpringer
dc.relation#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.relationdatasets
dc.relationEnvironmental monitoring and assessment
dc.rightsopen
dc.sourceEnvironmental Monitoring and Assessment 2013; 185(3):2565-2576
dc.subjectAdsorción
dc.subjectMonitoreo del Ambiente
dc.subjectLevivirus
dc.subjectLímite de Detección
dc.subjectPseudomonas aeruginosa
dc.subjectRíos
dc.subjectUltrafiltración
dc.subjectMicrobiología del Agua
dc.titleEvaluation of concentration efficiency of the Pseudomonas aeruginosa phage PP7 in various water matrixes by different methods
dc.typeArtículo


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