Evaluation of an enzyme-linked immunosorbent assay for detection of antibodies to Junin virus in rodents

dc.creatorMorales, María Alejandra
dc.creatorCalderón, Gladys
dc.creatorRiera, Laura
dc.creatorAmbrosio, Ana María
dc.creatorEnria, Delia
dc.creatorSabattini, Marta S.
dc.date2020-12-14T19:40:32Z
dc.date2020-12-14T19:40:32Z
dc.date2002-05
dc.date.accessioned2023-08-29T20:07:48Z
dc.date.available2023-08-29T20:07:48Z
dc.identifier0166-0934
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1892
dc.identifier10.1016/s0166-0934(01)00452-9
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519844
dc.descriptionFil: Morales, María A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Virales Humanas; Argentina.
dc.descriptionFil: Calderón, Gladys E. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Virales Humanas; Argentina.
dc.descriptionFil: Riera, Laura M. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Virales Humanas; Argentina.
dc.descriptionFil: Ambrosio, Ana M. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Virales Humanas; Argentina.
dc.descriptionFil: Enría, Delia A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Virales Humanas; Argentina.
dc.descriptionFil: Sabattini, Marta S. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Virales Humanas; Argentina.
dc.descriptionJunin virus is the etiological agent of Argentine hemorrhagic fever, a serious rodent-borne disease. An enzyme-linked immunosorbent assay (ELISA) to detect Junin virus IgG antibodies in rodents was evaluated using sera from 27 Calomys musculinus and five Calomys laucha, inoculated experimentally with a live attenuated strain of this arenavirus. The test performance was compared against an indirect immunofluorescence assay (IFA). The ELISA had a sensitivity and specificity of 100% and a reproducibility of 87.9% for samples with titers above the selected cut-off value. IFA had lower sensitivity (53%) with the same specificity. The ELISA results were similar, whether carried out on whole blood or serum samples, thus eliminating the need for serum separation. A high correlation (K=0.86) between ELISA and IFA results was obtained from 1011 wild sigmodontine and murine rodents collected within and outside of the Argentine hemorrhagic fever endemic area. These results indicate that Junin virus IgG ELISA is the most suitable assay for detection of Junin virus antibodies in rodent samples.
dc.languageen
dc.publisherElsevier
dc.relation#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.relationdatasets
dc.relationJournal of virological methods
dc.rightsnone
dc.sourceJournal of Virological Methods 2002; 103(1):57-66
dc.subjectAnimales
dc.subjectAnimales Salvajes
dc.subjectAnticuerpos Antivirales
dc.subjectInfecciones por Arenaviridae
dc.subjectChlorocebus aethiops
dc.subjectReservorios de Enfermedades
dc.subjectTécnica del Anticuerpo Fluorescente Indirecta
dc.subjectInmunoglobulina G
dc.subjectVirus Junin
dc.subjectMuridae
dc.subjectReproducibilidad de los Resultados
dc.subjectEnfermedades de los Roedores
dc.subjectSensibilidad y Especificidad
dc.subjectCélulas Vero
dc.subjectEnsayo de Inmunoadsorción Enzimática
dc.titleEvaluation of an enzyme-linked immunosorbent assay for detection of antibodies to Junin virus in rodents
dc.typeArtículo


Este ítem pertenece a la siguiente institución