Estudio retrospectivo de la aplicación de la amplificación génica cualitativa en muestras biológicas para el seguimiento de la toxoplasmosis en pacientes pediátricos receptores de trasplante de células progenitoras hematopoyéticas

Retrospective study of the implementation of the qualitative PCR technique in biological samples for monitoring toxoplasmosis in pediatric patients receiving hematopoietic stem cell transplantation

dc.creatorNigro, Mónica G
dc.creatorFigueroa, Carlos
dc.creatorLedesma, Bibiana A
dc.date2020-12-14T19:38:38Z
dc.date2020-12-14T19:38:38Z
dc.date2014-01
dc.date.accessioned2023-08-29T20:07:47Z
dc.date.available2023-08-29T20:07:47Z
dc.identifier0325-7541
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1885
dc.identifier10.1016/S0325-7541(14)70043-4
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519832
dc.descriptionFil: Nigro, Mónica G. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: Figueroa, Carlos. Hospital de Pediatría S.A.M.I.C. "Profesor Dr. Juan P. Garrahan". Servicio de Trasplante de Médula Ósea; Argentina.
dc.descriptionFil: Ledesma, Bibiana A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionToxoplasmosis is an opportunistic infection caused by the parasite Toxoplasma gondii. The infection is severe and difficult to diagnose in patients receiving allogeneic hematopoietic stem cell transplantation (HSCT). Twelve patients receiving HSCT were monitored post-transplant, by qualitative PCR at the Children's Hospital S.A.M.I.C. "Prof. Dr. Juan P. Garrahan". The monitoring of these patients was defined by a history of positive serology for toxoplasmosis in the donor or recipient and because their hematologic condition did not allow the use of trimethoprim-sulfamethoxazole for prophylaxis. During the patients' monitoring, two of them with positive PCR results showed signs of illness by T. gondii and were treated with pyrimethamine-clindamycin. In two other patients, toxoplasmosis was the cause of death and an autopsy finding, showing negative PCR results. Four patients without clinical manifestations received treatment for toxoplasmosis because of positive PCR detection. In four patients there were no signs of toxoplasmosis disease and negative PCR results during follow-up. The qualitative PCR technique proved useful for the detection of toxoplasmosis reactivation in HSCT recipients, but has limitations in monitoring and making clinical decisions due to the persistence of positive PCR over time and manifestations of toxicity caused by the treatment.
dc.formatpdf
dc.languagees
dc.publisherAsociación Argentina de Microbiología
dc.relation#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.relationdatasets
dc.relationRevista Argentina de microbiologia
dc.rightsopen
dc.sourceRevista Argentina de Microbiología 2014; 46(1):24-9
dc.subjectReacción en Cadena de la Polimerasa
dc.subjectToxoplasma
dc.subjectAdolescente
dc.subjectAloinjertos
dc.subjectAntiinfecciosos
dc.subjectAntiprotozoarios
dc.subjectNiño
dc.subjectPreescolar
dc.subjectClindamicina
dc.subjectContraindicaciones
dc.subjectADN Protozoario
dc.subjectReacciones Falso Negativas
dc.subjectReacciones Falso Positivas
dc.subjectFemenino
dc.subjectHumanos
dc.subjectHuésped Inmunocomprometido
dc.subjectInmunosupresores
dc.subjectMasculino
dc.subjectInfecciones Oportunistas
dc.subjectComplicaciones Posoperatorias
dc.subjectValor Predictivo de las Pruebas
dc.subjectPremedicación
dc.subjectPirimetamina
dc.subjectEstudios Retrospectivos
dc.subjectDonantes de Tejidos
dc.subjectToxoplasmosis
dc.subjectCombinación Trimetoprim y Sulfametoxazol
dc.subjectTrasplante de Células Madre Hematopoyéticas
dc.titleEstudio retrospectivo de la aplicación de la amplificación génica cualitativa en muestras biológicas para el seguimiento de la toxoplasmosis en pacientes pediátricos receptores de trasplante de células progenitoras hematopoyéticas
dc.titleRetrospective study of the implementation of the qualitative PCR technique in biological samples for monitoring toxoplasmosis in pediatric patients receiving hematopoietic stem cell transplantation
dc.typeArtículo


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