Epstein Barr virus genotypes and LMP-1 variants in HIV-infected patients

dc.creatorCorrea, Rita Mariel
dc.creatorFellner, María Dolores
dc.creatorDurand, Karina
dc.creatorRedini, Liliana
dc.creatorAlonio, Virginia
dc.creatorYampolsky, Claudio
dc.creatorColobraro, Antonio
dc.creatorSevlever, Gustavo
dc.creatorTeyssie, Angelica R.
dc.creatorBenetucci, Jorge
dc.creatorPicconi, María Alejandra
dc.date2020-12-10T14:30:16Z
dc.date2020-12-10T14:30:16Z
dc.date2007-04
dc.date.accessioned2023-08-29T20:07:42Z
dc.date.available2023-08-29T20:07:42Z
dc.identifier0146-6615
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1870
dc.identifier10.1002/jmv.20782
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519798
dc.descriptionFil: Correa, Rita Mariel. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: Fellner, María Dolores. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: Durand, Karina. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: Redini, Liliana. Hospital de Enfermedades Infecciosas “Dr. F. Muñiz”; Argentina.
dc.descriptionFil: Alonio, Virginia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: Yampolsky, Claudio. Sanatorio Güemes. Servicio de Neurocirugía; Argentina.
dc.descriptionFil: Colobraro, Antonio. Clínica Bazterrica. Servicio Patología; Argentina.
dc.descriptionFil: Sevlever, Gustavo. Instituto Fleni. Servicio Patología; Argentina.
dc.descriptionFil: Teyssié, Angélica. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: Benetucci, Jorge. Fundación Ayuda al Inmunodeficiente. Laboratorio de Retrovirus y virus asociados; Argentina.
dc.descriptionFil: Picconi, María Alejandra. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionTwo Epstein Barr virus (EBV) genotypes: EBV-1 and EBV-2 have been described. A 30-bp deletion in latent membrane protein-1 gene (del-LMP-1) has been identified in various pathologies. The aim of this study was to determine EBV genotypes and 30-bp deletion frequency in HIV-infected patients from Argentina. The study was performed on 258 individuals: Cases: 144 HIV-infected patients that included: (a) 7 AIDS patients with primary central nervous system lymphoma (PCNSL), (b) 62 AIDS patients, and (c) 75 asymptomatic HIV-infected patients. Controls: 114 HIV-negative individuals. EBV genotypes and variants in LMP-1 gene were detected by polymerase chain reaction (PCR)-Southern blot on DNA extracted from peripheral blood mononuclear cells and brain biopsies. In PCNSL, the presence of EBV was confirmed by EBER RNA in situ hybridization, and DNA sequencing of 3' end LMP-l gene of PCR products was performed. In HIV-infected patients, EBV-1 was detected in 48.6%, EBV-2 in 18.8%, and co-infection with both genotypes in 32.6%. In control group, EBV-1 was present in 74.3%, EBV-2 in 12.4%, and co-infection in 13.3%. Del-LMP-1 was found in 44.4% of HIV-infected patients samples (20.7% alone and 23.7% co-infection with non-deleted form) while it was found in 25.3% (6.3% alone and 19% with co-infection) in HIV-negative individuals. In HIV-infected patients EBV-2, co-infection and 30-bp deletion are more prevalent than in control group. In all, PCNSL brain biopsies samples, del-LMP-1 always was detected with EBV-2, but more cases would have to be included to draw definitive conclusions.
dc.languageen
dc.publisherWiley
dc.publisherWiley-Liss
dc.relation#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.relationdatasets
dc.relationJournal of medical virology
dc.rightsnone
dc.sourceJournal of Medical Virology 2007; 79(4):401-407
dc.subjectSíndrome de Inmunodeficiencia Adquirida
dc.subjectAdolescente
dc.subjectAdulto
dc.subjectArgentina
dc.subjectSecuencia de Bases
dc.subjectBiopsia
dc.subjectSouthern Blotting
dc.subjectEncéfalo
dc.subjectNeoplasias del Sistema Nervioso Central
dc.subjectInfecciones por Virus de Epstein-Barr
dc.subjectVariación Genética
dc.subjectInfecciones por VIH
dc.subjectHerpesvirus Humano 4
dc.subjectHumanos
dc.subjectLeucocitos Mononucleares
dc.subjectLinfoma Relacionado con SIDA
dc.subjectMasculino
dc.subjectPersona de Mediana Edad
dc.subjectReacción en Cadena de la Polimerasa
dc.subjectEliminación de Secuencia
dc.subjectEspecificidad de la Especie
dc.subjectProteínas de la Matriz Viral
dc.subjectVIH
dc.titleEpstein Barr virus genotypes and LMP-1 variants in HIV-infected patients
dc.typeArtículo


Este ítem pertenece a la siguiente institución