Primer aislamiento ambiental de Cryptococcus neoformans var. gattii en Argentina

1st environmental isolation of Cryptococcus neoformans var. gattii in Argentina

dc.creatorDavel, Graciela Odelsia
dc.creatorAbrantes, Ruben Antonio
dc.creatorBrudny, M
dc.creatorCórdoba, Susana
dc.creatorRodero, Laura
dc.creatorCanteros, Cristina
dc.creatorPerrotta, Diego
dc.date2020-12-10T14:25:13Z
dc.date2020-12-10T14:25:13Z
dc.date2003
dc.date.accessioned2023-08-29T20:07:38Z
dc.date.available2023-08-29T20:07:38Z
dc.identifier0325-7541
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1853
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519760
dc.descriptionFil: Davel, Graciela Odelsia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.
dc.descriptionFil: Abrantes, R. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.
dc.descriptionFil: Brudny, M. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.
dc.descriptionFil: Rodero, L. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.
dc.descriptionFil: Canteros, Cristina. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.
dc.descriptionTwo strains of C. neoformans var. gattii serotype B were isolated from a park in Buenos Aires city, Argentina. Samples were collected in spring, rubbing with swabs on the inner hollow of Eucalyptus spp. and other trees, which were not identified at the collection moment. Samples were swabbed directly onto cafeic acid with antibiotic agar, then incubated at 28 degrees C and observed daily for a week. Isolates were recovered from cafeic acid medium showing characteristic colonies of the species that allowed differentiation from other microorganisms of the sample. Identification was performed using carbohydrate fermentation, assimilation of nitrogen and carbon sources, and urease and phenoloxidase detection. Strain variety was determined with canavanine-glicine-bromotimol blue agar medium (CGB). Genotypic characterization using AP-PCR with (GACA)4 aleatory primer showed that both band profiles could be differentiated by only one band. These two strains were isolated from tree species different from Eucalyptus spp. These isolates from natural sources demonstrate that C. neoformans var. gattii efosts in Buenos Aires city environment and that it is not necessarily associated with Eucalyptus spp. The presence of C. neoformansvar. gattii in the environment, detected for the first time in Argentina, may explain its sporadic association with human infections in the local population.
dc.languagees
dc.relationRevista Argentina de microbiologia
dc.rightsopen
dc.sourceRevista Argentina de Microbiología 2003; 35(2): 110-2.
dc.subjectCryptococcus
dc.subjectCryptococcus neoformans
dc.subjectArgentina
dc.titlePrimer aislamiento ambiental de Cryptococcus neoformans var. gattii en Argentina
dc.title1st environmental isolation of Cryptococcus neoformans var. gattii in Argentina
dc.typeArtículo


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