Putative virulence factors and clonal relationship of O174 Shiga toxin-producing Escherichia coli isolated from human, food and animal sources

dc.creatorCundon, Cecilia
dc.creatorCarbonari, Carolina C
dc.creatorZolezzi, Gisela
dc.creatorRivas, Marta
dc.creatorBentancor, Adriana
dc.date2020-12-04T09:08:41Z
dc.date2020-12-04T09:08:41Z
dc.date2018-02
dc.date.accessioned2023-08-29T20:07:34Z
dc.date.available2023-08-29T20:07:34Z
dc.identifier0378-1135
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1817
dc.identifier10.1016/j.vetmic.2017.12.006
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519729
dc.descriptionFil: Cundon, Cecilia. Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Cátedra de Microbiología; Argentina.
dc.descriptionFil: Carbonari, Claudia C. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
dc.descriptionFil: Zolezzi, Gisela. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
dc.descriptionFil: Rivas, Marta. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
dc.descriptionFil: Bentancor, A. Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Cátedra de Microbiología; Argentina.
dc.descriptionShiga toxin-producing Escherichia coli (STEC) is th etiological agent of gastrointestinal diseases as haemorrhagic colitis and haemolytic-uraemic syndrome (HUS). Shiga toxin (Stx) is the main virulence factor. There are two types, Stx1 and Stx2, and several subtypes. Other virulence factors are involved in pathogenesis. While O157:H7 is the predominant serotype, at present non-O157 STEC strains are increasingly recognized as foodborne pathogens worldwide. In Argentina, STEC O174 stands out as an emerging pathogen and is one of the four most prevalent non-O157 STEC serogroups. The aim of this study was to characterize 41 O174:[H21, H28] STEC strains isolated from animals, food, and humans. Isolates were characterized by stx genotyping, adhesion factors (afaC, eae, iha, lpfO113, saa, and toxB), additional toxins (cdtV, ehxA, subA) and clonal relationship by pulsed-field gel electrophoresis (PFGE). Among 30 O174:H21 strains, the most prevalent stx subtype was stx2c (56.7%), and among 11 O174:H28 strains, the most prevalent was stx2a (90.9%). Regarding virulence factors, all strains were positive for afaC gene and negative for eae and toxB genes. In O174:H21, the frequency of additional factors was lpfO113 (96.6%), iha (83.3%), ehxA and saa (10%), and subA (3.3%), meanwhile in O174:H28 strains the frequency was iha and subA (100%), lpfO113 (90.9%), ehxA and saa (90.9%), and cdtV (9.09%). By Xbal-PFGE, 29 patterns were established with 64.01% similarity and three clusters were detected. Given the fact that the O174 serogroup is a local emergent, it is important to study the virulence profiles to understand its potential pathogenicity.
dc.formatpdf
dc.languageen
dc.relationVeterinary microbiology
dc.rightsnone
dc.sourceVeterinary Microbiology 2018; 215:29-34.
dc.subjectElectroforesis en Gel de Agar
dc.subjectVirulencia
dc.subjectEscherichia coli
dc.titlePutative virulence factors and clonal relationship of O174 Shiga toxin-producing Escherichia coli isolated from human, food and animal sources
dc.typeArtículo


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