Virulence profile comparison between LEE-negative Shiga toxin-producing Escherichia coli (STEC) strains isolated from cattle and humans

dc.creatorGalli, Lucía
dc.creatorMiliwebsky, Elizabeth
dc.creatorIrino, Kinue
dc.creatorLeotta, Gerardo A
dc.creatorRivas, Marta
dc.date2020-12-04T09:12:45Z
dc.date2020-12-04T09:12:45Z
dc.date2010-07-14
dc.date.accessioned2023-08-29T20:07:34Z
dc.date.available2023-08-29T20:07:34Z
dc.identifier0378-1135
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1829
dc.identifier10.1016/j.vetmic.2009.11.028
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519726
dc.descriptionFil: Galli, Lucía. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
dc.descriptionFil: Miliwebsky, Elizabeth. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
dc.descriptionFil: Irino, Kinue. Departamento de Microbiologia, Setor de Enterobactérias, Instituto Adolfo Lutz; Brasil.
dc.descriptionFil: Leotta, Gerardo A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
dc.descriptionFil: Rivas, Marta. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
dc.descriptionFor comparison purposes, the prevalence of 8 virulence markers was investigated, by PCR, in 153 cattle and 47 human Locus for Enterocyte Effacement (LEE)-negative Shiga toxin-producing Escherichia coli (STEC) strains isolated in Argentina. Also, their correlation with severe disease was established. The virulence markers studied comprises 5 fimbrial and nonfimbrial adhesin-encoding genes (fimA, iha, efa1, lpfA(O113), and saa) and 3 toxin genes (cdt-V, subAB and astA) in addition to the Shiga toxins. The most prevalent virulence marker found was that encoded by the lpfA(O113) gene (199/200, 99%). Comparatively, the lpfA(O113), fimA, iha, saa, subAB, cdt-V and astA genes were detected in 100%, 92.8%, 85%, 52.9%, 36%, 11.8% and 9.8% of the cattle strains and in 97.9%, 95.7%, 89.4%, 40.4%, 32%, 17% and 10.6% of the human strains, respectively. All STEC strains were efa1 negative. The most prevalent profile observed among cattle and human STEC strains was lpfA(O113)iha fimA. These results show that bovine LEE-negative STEC strains possessed genes encoding virulence factors present in human LEE-negative STEC strains that are associated with disease. Despite a great diversity of virulence profiles observed, further studies comparing wild type strains and their allelic mutants are needed to evaluate the role of each factor in the pathogenesis of LEE-negative STEC strains during human infections.
dc.languageen
dc.publisherElsevier
dc.relationVeterinary microbiology
dc.rightsnone
dc.sourceVeterinary Microbiology 2010; 143(2-4):307-13
dc.subjectVirulencia
dc.subjectToxina Shiga
dc.subjectEscherichia coli
dc.titleVirulence profile comparison between LEE-negative Shiga toxin-producing Escherichia coli (STEC) strains isolated from cattle and humans
dc.typeArtículo


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