Use of Brucella canis antigen for detection of ovine serum antibodies against Brucella ovis

dc.creatorLópez, G.
dc.creatorAyala, Sandra M.
dc.creatorEscobar, Gabriela I.
dc.creatorLucero, Nidia E.
dc.date2020-12-04T09:11:41Z
dc.date2020-12-04T09:11:41Z
dc.date2005-02-25
dc.date.accessioned2023-08-29T20:07:33Z
dc.date.available2023-08-29T20:07:33Z
dc.identifier0378-1135
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1826
dc.identifier10.1016/j.vetmic.2004.10.020
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519720
dc.descriptionFil: Lopez, G. Facultad de Ciencias Agrarias, Universidad Nacional de Lomas de Zamora; Argentina
dc.descriptionFil: Ayala, Sandra M. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: Escobar, Gabriela I. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: Lucero, Nidia E. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionBrucella ovis causes a genital disease of sheep manifested by epididymitis in rams and placentitis in ewes producing reduced fertility in the flock. Clinical diagnosis is not sensitive enough and bacteriological testing is not feasible for detection of the disease in large numbers of animals. Indirect methods of serological testing are preferred for routine diagnosis, of which agar gel immunodiffusion (AGID), complement fixation (CF) and ELISA tests are recommended as the most efficient. Since B. ovis shares antigenic components with Brucella canis, it would seem that either strain could be used as antigen with the same results; however, the advantage of the B. canis (M-) strain variant is that it can be used to develop a satisfactory antigen for agglutination tests. We present data on AGID and IELISA tests using B. ovis antigen and rapid screening agglutination test (RSAT), 2-mercapto-ethanol RSAT (2ME-RSAT) and IELISA using B. canis antigen. We tested 225 animals. The cut-off values were adjusted by ROC analysis using 51 negative and 32 positive sera; the IELISA-B. canis cut-off value was 39 (%P) and IELISA-B. ovis, 51 (%P), with 100% sensitivity and specificity. Of the 32 positive sera from the infected flock RSAT detected 32 (100%), 2ME-RSAT 29 (91%) and AGID 31 (97%). Of the 142 sera from suspicious flocks, 46 were negative and 56 positive in all the tests; 16 were positive by RSAT, IELISA-B. canis and IELISA-B. ovis, 20 positive only with RSAT and 2 positive only by both IELISAs. RSAT is a very sensitive screening test that, because of its simplicity and easy interpretation, following a study in larger sample, could replace AGID as a screening test for diagnosis of ovine brucellosis caused by B. ovis. The IELISA-B. canis or IELISA-B. ovis could be used as confirmatory tests, since they show equal specificity and sensitivity.
dc.formatpdf
dc.languageen
dc.publisherElsevier
dc.relationVeterinary microbiology
dc.rightsnone
dc.sourceVeterinary Microbiology 2005; 105 (3-4): 181-7.
dc.subjectBrucella
dc.subjectBrucelosis
dc.subjectAnticuerpos
dc.titleUse of Brucella canis antigen for detection of ovine serum antibodies against Brucella ovis
dc.typeArtículo


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