Emerging types of Shiga toxin-producing E. coli (STEC) O178 present in cattle, deer, and humans from Argentina and Germany

dc.creatorMiko, Angelika
dc.creatorRivas, Marta
dc.creatorBentancor, Adriana
dc.creatorDelannoy, Sabine
dc.creatorFach, Patrick
dc.creatorBeutin, Lothar
dc.date2020-12-04T09:09:23Z
dc.date2020-12-04T09:09:23Z
dc.date2014-06
dc.date.accessioned2023-08-29T20:07:32Z
dc.date.available2023-08-29T20:07:32Z
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1819
dc.identifier10.3389/fcimb.2014.00078
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519706
dc.descriptionFil: Miko, Angelika. Federal Institute for Risk Assessment. National Reference Laboratory for Escherichia coli. Division of Microbial Toxins; Alemania.
dc.descriptionFil: Rivas, Marta. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: Bentancor, Adriana. Universidad de Buenos Aires Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Microbiología; Argentina.
dc.descriptionFil: Delannoy, Sabine. French Agency for Food, Environmental and Occupational Health. Food Safety Laboratory; Francia.
dc.descriptionFil: Fach, Patrick. French Agency for Food, Environmental and Occupational Health. Food Safety Laboratory; Francia.
dc.descriptionFil: Beutin, Lothar. Federal Institute for Risk Assessment. National Reference Laboratory for Escherichia coli. Division of Microbial Toxins; Alemania.
dc.descriptionMore than 400 serotypes of Shiga toxin-producing Escherichia coli (STEC) have been implicated in outbreaks and sporadic human diseases. In recent years STEC strains belonging to serogroup O178 have been commonly isolated from cattle and food of bovine origin in South America and Europe. In order to explore the significance of these STEC strains as potential human pathogens, 74 German and Argentinean E. coli O178 strains from animals, food and humans were characterized phenotypically and investigated for their serotypes, stx-genotypes and 43 virulence-associated markers by a real-time PCR-microarray. The majority (n = 66) of the O178 strains belonged to serotype O178:H19. The remaining strains divided into O178:H7 (n = 6), O178:H10 (n = 1), and O178:H16 (n = 1). STEC O178:H19 strains were mainly isolated from cattle and food of bovine origin, but one strain was from a patient with hemolytic uremic syndrome (HUS). Genotyping of the STEC O178:H19 strains by pulsed-field gel electrophoresis revealed two major clusters of genetically highly related strains which differ in their stx-genotypes and non-Stx putative virulence traits, including adhesins, toxins, and serine-proteases. Cluster A-strains including the HUS-strain (n = 35) carried genes associated with severe disease in humans (stx2a, stx2d, ehxA, saa, subAB1, lpfAO113 , terE combined with stx1a, espP, iha). Cluster B-strains (n = 26) showed a limited repertoire of virulence genes (stx2c, pagC, lpfAO113 , espP, iha). Among O178:H7 strains isolated from deer meat and patients with uncomplicated disease a new STEC variant was detected that is associated with the genotype stx1c/stx2b/ehxA/subAB2/espI/[terE]/espP/iha. None of the STEC O178 strains was positive for locus of enterocyte effacement (LEE)- and nle-genes. Results indicate that STEC O178:H19 strains belong to the growing group of LEE-negative STEC that should be considered with respect to their potential to cause diseases in humans.
dc.formatpdf
dc.languageen
dc.publisherFrontiers Media
dc.relation#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.relationdatasets
dc.relationFrontiers in cellular and infection microbiology
dc.rightsopen
dc.sourceFrontiers in Cellular and Infection Microbiology 2014; 4(78):1-14
dc.subjectEscherichia coli Shiga-Toxigénica
dc.subjectToxinas Shiga
dc.subjectVirulencia
dc.subjectAnimales
dc.subjectArgentina
dc.subjectBovinos
dc.subjectAnálisis por Conglomerados
dc.subjectADN Bacteriano
dc.subjectCiervos
dc.subjectElectroforesis en Gel de Campo Pulsado
dc.subjectInfecciones por Escherichia coli
dc.subjectProteínas de Escherichia coli
dc.subjectGenotipo
dc.subjectAlemania
dc.subjectHumanos
dc.subjectDatos de Secuencia Molecular
dc.subjectReacción en Cadena de la Polimerasa
dc.subjectAnálisis de Secuencia de ADN
dc.subjectSerotipificación
dc.subjectFactores de Virulencia
dc.subjectMicrobiología de Alimentos
dc.titleEmerging types of Shiga toxin-producing E. coli (STEC) O178 present in cattle, deer, and humans from Argentina and Germany
dc.typeArtículo


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