Amino acid substitution in Cryptococcus neoformans lanosterol 14-α-demethylase involved in fluconazole resistance in clinical isolates

dc.creatorBosco-Borgeat, María Eugenia
dc.creatorMazza, Mariana
dc.creatorTaverna, Constanza Giselle
dc.creatorCórdoba, Susana
dc.creatorMurisengo, Omar Alejandro
dc.creatorVivot, Walter
dc.creatorDavel, Graciela Odelsia
dc.date2020-12-04T09:07:59Z
dc.date2020-12-04T09:07:59Z
dc.date2016
dc.date.accessioned2023-08-29T20:07:31Z
dc.date.available2023-08-29T20:07:31Z
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1815
dc.identifierhttps://doi.org/10.1016/j.ram.2016.03.003
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519701
dc.descriptionFil: Bosco-Borgeat, María E. ANLIS Dr. C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento Micología; Argentina.
dc.descriptionFil: Mazza, Mariana. ANLIS Dr. C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento Micología; Argentina.
dc.descriptionFil: Taverna, Constanza Giselle. ANLIS Dr. C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento Micología; Argentina.
dc.descriptionFil: Córdoba, Susana. ANLIS Dr. C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento Micología; Argentina.
dc.descriptionFil: Murisengo, Omar A. ANLIS Dr. C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento Micología; Argentina.
dc.descriptionFil: Vivot, Walter. ANLIS Dr. C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento Micología; Argentina.
dc.descriptionFil: Davel, Graciela Odelsia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Micología; Argentina.
dc.descriptionThe molecular basis of fluconazole resistance in Cryptococcus neoformans has been poorly studied. A common azole resistance mechanism in Candida species is the acquisition of point mutations in the ERG11 gene encoding the enzyme lanosterol 14-α-demethylase, target of the azole class of drugs. In C. neoformans only two mutations were described in this gene. In order to evaluate other mutations that could be implicated in fluconazole resistance in C. neoformans we studied the genomic sequence of the ERG11 gene in 11 clinical isolates with minimal inhibitory concentration (MIC) values to fluconazole of ≥16μg/ml. The sequencing revealed the G1855A mutation in 3 isolates, resulting in the enzyme amino acid substitution G484S. These strains were isolated from two fluconazole-treated patients. This mutation would not intervene in the susceptibility to itraconazole and voriconazole.
dc.formatpdf
dc.languageen
dc.relationRevista Argentina de microbiologia
dc.rightsopen
dc.subjectMutación Missense
dc.subjectMeningitis Criptocócica
dc.subjectProteínas Fúngicas
dc.subjectFarmacorresistencia Fúngica
dc.subjectAntifúngicos
dc.titleAmino acid substitution in Cryptococcus neoformans lanosterol 14-α-demethylase involved in fluconazole resistance in clinical isolates
dc.typeArtículo


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