Triton Hodge Test: Improved Protocol for Modified Hodge Test for Enhanced Detection of NDM and Other Carbapenemase Producers

dc.creatorPasteran, Fernando
dc.creatorGonzalez, Lisandro J
dc.creatorAlbornoz, Ezequiel
dc.creatorBahr, Guillermo
dc.creatorVila, Alejandro J.
dc.creatorCorso, Alejandra
dc.date2020-12-02T14:29:50Z
dc.date2020-12-02T14:29:50Z
dc.date2016-03
dc.date.accessioned2023-08-29T20:07:28Z
dc.date.available2023-08-29T20:07:28Z
dc.identifier1098-660X
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1798
dc.identifier10.1128/JCM.01298-15
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519666
dc.descriptionFil: Pasteran, Fernando. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
dc.descriptionFil: Gonzalez, Lisandro J. Instituto de Biología Molecular y Celular de Rosario, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario; Argentina.
dc.descriptionFil: Albornoz, Ezequiel. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
dc.descriptionFil: Bahr, Guillermo. Instituto de Biología Molecular y Celular de Rosario, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario; Argentina.
dc.descriptionFil: Vila, Alejandro J. Instituto de Biología Molecular y Celular de Rosario, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario; Argentina.
dc.descriptionFil: Corso, Alejandra. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Antimicrobianos; Argentina.
dc.descriptionAccurate detection of carbapenemase-producing Gram-negative bacilli is of utmost importance for the control of nosocomial spread and the initiation of appropriate antimicrobial therapy. The modified Hodge test (MHT), a carbapenem inactivation assay, has shown poor sensitivity in detecting the worldwide spread of New Delhi metallo-β-lactamase (NDM). Recent studies demonstrated that NDM is a lipoprotein anchored to the outer membrane in Gram-negative bacteria, unlike all other known carbapenemases. Here we report that membrane anchoring of β-lactamases precludes detection of carbapenemase activity by the MHT. We also show that this limitation can be overcome by the addition of Triton X-100 during the test, which allows detection of NDM. We propose an improved version of the assay, called the Triton Hodge test (THT), which allows detection of membrane-bound carbapenemases with the addition of this nonionic surfactant. This test was challenged with a panel of 185 clinical isolates (145 carrying known carbapenemase-encoding genes and 40 carbapenemase nonproducers). The THT displayed test sensitivity of >90% against NDM-producing clinical isolates, while improving performance against other carbapenemases. Ertapenem provided the highest sensitivity (97 to 100%, depending on the type of carbapenemase), followed by meropenem (92.5 to 100%). Test specificity was not affected by the addition of Triton (87.5% and 92.5% with ertapenem and meropenem, respectively). This simple inexpensive test confers a large improvement to the sensitivity of the MHT for the detection of NDM and other carbapenemases.
dc.formatpdf
dc.languageen
dc.publisherAmerican Society for Microbiology
dc.relationJournal of clinical microbiology
dc.rightsopen
dc.sourceJournal of Clinical Microbiology 2016; 54(3):640-649.
dc.subjectEnterobacteriaceae
dc.titleTriton Hodge Test: Improved Protocol for Modified Hodge Test for Enhanced Detection of NDM and Other Carbapenemase Producers
dc.typeArtículo


Este ítem pertenece a la siguiente institución