Distribution of HPV-16 variants among isolates from Paraguayan women with different grades of cervical lesion

dc.creatorMendoza, Laura Patricia
dc.creatorPicconi, María Alejandra
dc.creatorMirazo, Santiago
dc.creatorMongelos, Pamela
dc.creatorGimenez, Graciela
dc.creatorBasiletti, Jorge
dc.creatorArbiza, Juan
dc.date2020-12-01T00:17:25Z
dc.date2020-12-01T00:17:25Z
dc.date2013-07
dc.date.accessioned2023-08-29T20:07:27Z
dc.date.available2023-08-29T20:07:27Z
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1784
dc.identifier10.1016/j.ijgo.2013.01.020
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519656
dc.descriptionFil: Mendoza, Laura. National University of Asunción. Department of Public Health and Epidemiology. Health Sciences Research Institute; Paraguay.
dc.descriptionFil: Picconi, María A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: Mirazo, Santiago. University of the Republic. Faculty of Sciences. Virology Section; Uruguay.
dc.descriptionFil: Mongelós, Pamela. National University of Asunción. Department of Public Health and Epidemiology. Health Sciences Research Institute; Paraguay.
dc.descriptionFil: Giménez, Graciela. National University of Asunción. Department of Public Health and Epidemiology. Health Sciences Research Institute; Paraguay.
dc.descriptionFil: Basiletti, Jorge. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: Arbiza, Juan. University of the Republic. Faculty of Sciences. Virology Section; Uruguay.
dc.descriptionObjective: To determine the distribution of HPV-16 variants among Paraguayan women with different grades of cervical lesions. Methods: Sixty-seven HPV-16-positive cervical samples obtained from women attending health centers in Paraguay between March 2007 and April 2009 were examined, including 29 low-grade squamous intraepithelial lesion (LSIL), 29 high-grade squamous intraepithelial lesion (HSIL), 4 cervical cancer, and 5 normal cytology samples. The specimens were analyzed by PCR-directed sequencing of a 364-bp fragment of the long control region of HPV-16, and a phylogenetic tree was compiled with MEGA 5.0 software. Results: Most HPV-16 variants belonged to the European branch (82%); these variants were detected among 25 of 29 women with LSIL, 22 of 29 women with HSIL, 3 of 4 women with cervical cancer, and all women with normal cytology. Two isolates yielded new variants of the European branch with nucleotide substitutions at positions A7752C and A7810T. Non-European variants, such as African type 1 (1.5%) and Asian-American (16.5%), were detected only among women with cervical lesions (4/29, LSIL; 6/29, HSIL; 1/29, cervical cancer). These variants had at least 6 nucleotide substitutions adjacent to or within transcription factor binding sites. Conclusion: All branches of HPV-16 variants were detected among Paraguayan women with cervical lesions.
dc.languageen
dc.publisherElsevier
dc.relation#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.relationdatasets
dc.relationInternational journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics
dc.rightsnone
dc.sourceInternational Journal of Gynecology & Obstetrics 2013; 122(1):44-7
dc.subjectAdolescente
dc.subjectAdulto
dc.subjectAnciano
dc.subjectSitios de Unión
dc.subjectNeoplasia Intraepitelial Cervical
dc.subjectFemenino
dc.subjectPapillomavirus Humano 16
dc.subjectHumanos
dc.subjectPersona de Mediana Edad
dc.subjectNucleótidos
dc.subjectInfecciones por Papillomavirus
dc.subjectParaguay
dc.subjectReacción en Cadena de la Polimerasa
dc.subjectAnálisis de Secuencia de ADN
dc.subjectFactores de Transcripción
dc.subjectNeoplasias del Cuello Uterino
dc.subjectAdulto Joven
dc.titleDistribution of HPV-16 variants among isolates from Paraguayan women with different grades of cervical lesion
dc.typeArtículo


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