Downregulation of connexin 43 gene expression in rat heart during inflammation. The role of tumour necrosis factor

dc.creatorFernández Cobo, Mariana
dc.creatorGingalewski, Cynthia
dc.creatorDrujan, Doreen
dc.creatorDe Maio, Antonio
dc.date2020-12-01T00:14:22Z
dc.date2020-12-01T00:14:22Z
dc.date1999-03
dc.date.accessioned2023-08-29T20:07:26Z
dc.date.available2023-08-29T20:07:26Z
dc.identifier1043-4666
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1781
dc.identifier10.1006/cyto.1998.0422
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519651
dc.descriptionFil: Fernández-Cobo, Mariana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: Gingalewski, Cynthia. Johns Hopkins University. School of Medicine. Division of Pediatric Surgery; Estados Unidos.
dc.descriptionFil: Drujan, Doreen. Johns Hopkins University. School of Medicine. Division of Pediatric Surgery; Estados Unidos.
dc.descriptionFil: De Maio, Antonio. Johns Hopkins University. School of Medicine. Division of Pediatric Surgery; Estados Unidos.
dc.descriptionGap junctions form channels that mediate the communication between adjacent cells. Alterations in gap junction function and/or expression are believed to contribute to cardiac dysfunction such as those observed in septic patients. The expression of connexin 43 (Cx43), the subunit component of the most abundant cardiac gap junction, was analysed in rat heart during inflammation induced by the administration of bacterial lipopolysaccharide (LPS). Cx43 mRNA levels were found to be dramatically (>50%) and rapidly (2 h) reduced in the heart after injection of LPS (1 mg/kg). To investigate the possible mechanism of the decrease in Cx43 expression during inflammation, the promoter region of this gene was cloned. The basal Cx43 promoter activity was observed within 224-134 bp of the transcriptional initiation site after transfection into a rat myoblast cell-line (H9c2). The Cx43 promoter activity was found to be reduced by incubation of the transfected cells with serum obtained from LPS-treated rats. Moreover, Cx43 promoter activity was also decreased upon incubation with tumour necrosis factor alpha. These results suggest that Cx43 expression in the heart can be modulated by circulating cytokines. These observations may have important implications in the depression of heart function observed in septic patients.
dc.languageen
dc.relationCytokine
dc.rightsnone
dc.sourceCytokine 1999; 11(3):216-24
dc.subjectUniones Comunicantes
dc.subjectExpresión Génica
dc.subjectInflamación
dc.subjectMioblastos
dc.subjectSepsis
dc.titleDownregulation of connexin 43 gene expression in rat heart during inflammation. The role of tumour necrosis factor
dc.typeArtículo


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