Echinococcus granulosus: variability of the host-protective EG95 vaccine antigen in G6 and G7 genotypic variants

dc.creatorChow, Conan
dc.creatorGauci, Charles G
dc.creatorVural, Gulay
dc.creatorJenkins, David J
dc.creatorHeath, David D
dc.creatorRosenzvit, Mara C.
dc.creatorHarandi, Majid Fasihi
dc.creatorLightowlers, Marshall W
dc.date2020-11-24T23:07:55Z
dc.date2020-11-24T23:07:55Z
dc.date2008-08
dc.date.accessioned2023-08-29T20:07:14Z
dc.date.available2023-08-29T20:07:14Z
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1733
dc.identifier10.1016/j.exppara.2008.01.004
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519559
dc.descriptionFil: Chow, Conan. University of Melbourne. Veterinary Clinical Centre; Australia.
dc.descriptionFil: Gauci, Charles G. University of Melbourne. Veterinary Clinical Centre; Australia.
dc.descriptionFil: Vural, Gulay. Veterinary Control and Research Institute; Turquía.
dc.descriptionFil: Jenkins, David J. Hydatid Epidemiology and Control Program; Australia.
dc.descriptionFil: Heath, David D. Wallaceville Animal Research Centre; Nueva Zelanda.
dc.descriptionFil: Rosenzvit, Mara C. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: Harandi, Majid Fasihi. Kerman University of Medical Sciences. School of Medicine. Department of Parasitology; Iran.
dc.descriptionFil: Lightowlers, Marshall W. University of Melbourne. Veterinary Clinical Centre; Australia.
dc.descriptionCystic hydatid disease in humans is caused by the zoonotic parasite Echinococcus granulosus. As an aid to control transmission of the parasite, a vaccine has been produced for prevention of infection in the parasite's natural animal intermediate hosts. The vaccine utilizes the recombinant oncosphere protein, EG95. An investigation into the genetic variability of EG95 was undertaken in this study to assess potential antigenic variability in E. granulosus with respect to this host-protective protein. Gene-specific PCR conditions were first established to preferentially amplify the EG95 vaccine-encoding gene (designated eg95-1) from the E. granulosus genome that also contains several other EG95-related genes. The optimized PCR conditions were used to amplify eg95-1 from several parasite isolates in order to determine the protein-coding sequence of the gene. An identical eg95-1 gene was amplified from parasites showing a G1 or G2 genotype of E. granulosus. However, from isolates having a G6 or G7 genotype, a gene was amplified which had substantial nucleotide substitutions (encoding amino acid substitutions) compared with the eg95 gene family members. The amino acid substitutions of EG95 in the G6/G7 genotypes may affect the antigenicity/efficacy of the EG95 recombinant antigen against parasites of these genotypes. These findings indicate that characterization of eg95 gene family members in other strains/isolates of E. granulosus may provide valuable information about the potential for the EG95 hydatid vaccine to be effective against E. granulosus strains other than the G1 genotype.
dc.languageen
dc.publisherElsevier
dc.relation#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.relation#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.relationSistema de Gestión del Conocimiento de la Administración Nacional de Laboratorios e Institutos de Salud (ANLIS)
dc.relationdatasets
dc.relationExperimental parasitology
dc.rightsnone
dc.sourceExperimental Parasitology 2008; 119(4):499-505
dc.subjectSecuencia de Aminoácidos
dc.subjectAnimales
dc.subjectAntígenos Helmínticos
dc.subjectSouthern Blotting
dc.subjectADN de Helmintos
dc.subjectPerros
dc.subjectEquinococosis
dc.subjectEchinococcus granulosus
dc.subjectVariación Genética
dc.subjectGenotipo
dc.subjectProteínas del Helminto
dc.subjectHumanos
dc.subjectDatos de Secuencia Molecular
dc.subjectFamilia de Multigenes
dc.subjectReacción en Cadena de la Polimerasa
dc.subjectOvinos
dc.subjectEnfermedades de las Ovejas
dc.subjectPorcinos
dc.subjectVacunas Sintéticas
dc.subjectZoonosis
dc.titleEchinococcus granulosus: variability of the host-protective EG95 vaccine antigen in G6 and G7 genotypic variants
dc.typeArtículo


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