Echinococcus granulosus antigen B gene family: further studies of strain polymorphism at the genomic and transcriptional levels

dc.creatorMuzulin, Patricia Marcela
dc.creatorKamenetzky, Laura
dc.creatorGutierrez, Ariana Marcela
dc.creatorGuarnera, Eduardo
dc.creatorRosenzvit, Mara C.
dc.date2020-11-24T23:07:36Z
dc.date2020-11-24T23:07:36Z
dc.date2008-02
dc.date.accessioned2023-08-29T20:07:14Z
dc.date.available2023-08-29T20:07:14Z
dc.identifier0014-4894
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1732
dc.identifier10.1016/j.exppara.2007.07.004
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519555
dc.descriptionFil: Muzulin, Patricia Marcela. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: Kamenetzky, Laura. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: Gutiérrez, Ariana Marcela. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: Guarnera, Eduardo Alfredo. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionFil: Rosenzvit, Mara Cecilia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas; Argentina.
dc.descriptionEchinococcus granulosus AgB gene family is constituted by five gene loci. In a previous study, we analyzed the strain variation of EgAgB2/B4 sequences. Here, we have analyzed, by SSCP and sequencing, 250 genomic clones of EgAgB1/B3/B5 gene cluster from five E. granulosus strains. Several new EgAgB genomic variants were found. EgAgB1 and EgAgB3 genomic sequences grouped E. granulosus strains by phylogenetic tools in two clusters: one formed by G1/G2 and the other by G5, G6/G7 strains, in accordance with other molecular markers. EgAgB5 genomic and cDNA sequences were only found in G1/G2 cluster. Reverse transcription-PCR analysis using subunit specific primers revealed that all the EgAgB genes were transcribed in G1 and G7 strains with the exception of EgAgB5 transcripts that were not detected in G7 strain. Interestingly, AgB2 transcripts that were probably processed by an aberrant splicing mechanism leading to a non-functional B2 protein were found in G7 strain.
dc.languageen
dc.publisherElsevier
dc.relation#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.relationdatasets
dc.relationExperimental parasitology
dc.rightsnone
dc.sourceExperimental Parasitology 2008; 118(2):156-164
dc.subjectSecuencia de Aminoácidos
dc.subjectAnimales
dc.subjectSecuencia de Bases
dc.subjectCamelus
dc.subjectBovinos
dc.subjectAnálisis por Conglomerados
dc.subjectADN Complementario
dc.subjectADN de Helmintos
dc.subjectEquinococosis
dc.subjectEchinococcus granulosus
dc.subjectLipoproteínas
dc.subjectDatos de Secuencia Molecular
dc.subjectFamilia de Multigenes
dc.subjectFilogenia
dc.subjectPolimorfismo Conformacional Retorcido-Simple
dc.subjectReacción en Cadena de la Polimerasa de Transcriptasa Inversa
dc.subjectAlineación de Secuencia
dc.subjectOvinos
dc.subjectPorcinos
dc.subjectPolimorfismo Genético
dc.titleEchinococcus granulosus antigen B gene family: further studies of strain polymorphism at the genomic and transcriptional levels
dc.typeArtículo


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