Do commercial serologic tests for Trypanosoma cruzi infection detect Mexican strains in women and newborns?

dc.creatorGamboa-León, Miriam
dc.creatorGonzalez-Ramirez, Claudia
dc.creatorPadilla-Raygoza, Nicolás
dc.creatorSosa-Estani, Sergio
dc.creatorCaamal-Kantun, Alejandra
dc.creatorBuekens, Pierre
dc.creatorDumonteil, Eric
dc.date2020-11-24T12:51:45Z
dc.date2020-11-24T12:51:45Z
dc.date2011-04
dc.date.accessioned2023-08-29T20:07:09Z
dc.date.available2023-08-29T20:07:09Z
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1706
dc.identifier10.1645/GE-2545.1
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519512
dc.descriptionFil: Gamboa-León, Rubi. Tulane University. School of Public Health and Tropical Medicine; Estados Unidos.
dc.descriptionFil: González-Ramírez, Claudia. Universidad de Guanajuato. División Ciencias de la Salud e Ingenierías. Departamento de Enfermería y Obstetricia; México.
dc.descriptionFil: Padilla-Raygoza, Nicolás. Universidad de Guanajuato. División Ciencias de la Salud e Ingenierías. Departamento de Enfermería y Obstetricia; México.
dc.descriptionFil: Sosa-Estani, Sergio. ANLIS Dr.C.G.Malbrán. Centro Nacional de Diagnóstico e Investigación de Endemo-Epidemias; Argentina.
dc.descriptionFil: Caamal-Kantun, Alejandra. Tulane University. School of Public Health and Tropical Medicine; Estados Unidos.
dc.descriptionFil: Buekens, Pierre. Tulane University. School of Public Health and Tropical Medicine; Estados Unidos.
dc.descriptionFil: Dumonteil, Eric. Tulane University. School of Public Health and Tropical Medicine; Estados Unidos.
dc.descriptionWe sought to determine the serological test that could be used for Trypanosoma cruzi seroprevalence studies in Mexico, where lineage I predominates. In a previous study among pregnant women and their newborns in the states of Yucatan and Guanajuato, we reported a 0.8-0.9% of prevalence for T. cruzi -specific antibodies by Stat-Pak and Wiener ELISA. We have expanded this study here by performing an additional non-commercial ELISA and confirming the seropositives with Western blot, using whole antigens of a local parasite strain. We found a seroprevalence of 0.6% (3/500) in Merida and 0.4% in Guanajuato (2/488). The 5 seropositive umbilical cord samples reacted to both non-commercial ELISA and Western blot tests, and only 1 of the maternal samples was not reactive to non-commercial ELISA. A follow-up of the newborns at 10 mo was performed in Yucatan to determine the presence of T. cruzi antibodies in children as evidence of congenital infection. None of the children was seropositive. One newborn from an infected mother died at 2 wk of age of cardiac arrest, but T. cruzi infection was not confirmed. The T. cruzi seroprevalence data obtained with both commercial tests (Stat-Pak and ELISA Wiener) are similar to those from non-commercial tests using a local Mexican strain of T. cruzi.
dc.formatpdf
dc.languageen
dc.publisherAmerican Society of Parasitologists
dc.relation#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.relationdatasets
dc.relationThe Journal of parasitology
dc.rightsopen
dc.sourceJournal of Parasitology 2011; 97(2): 338–343
dc.subjectAdulto
dc.subjectAnticuerpos Antiprotozoarios
dc.subjectAntígenos de Protozoos
dc.subjectWestern Blotting
dc.subjectEnfermedad de Chagas
dc.subjectEstudios Transversales
dc.subjectEnsayo de Inmunoadsorción Enzimática
dc.subjectSangre Fetal
dc.subjectHumanos
dc.subjectRecién Nacido
dc.subjectTransmisión Vertical de Enfermedad Infecciosa
dc.subjectMéxico
dc.subjectEmbarazo
dc.subjectComplicaciones Parasitarias del Embarazo
dc.subjectSensibilidad y Especificidad
dc.subjectEstudios Seroepidemiológicos
dc.subjectTrypanosoma cruzi
dc.subjectAdulto Joven
dc.titleDo commercial serologic tests for Trypanosoma cruzi infection detect Mexican strains in women and newborns?
dc.typeArtículo


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