A myotoxic Lys49 phospholipase A2-homologue is the major component of the venom of Bothrops cotiara from Misiones, Argentina

dc.creatorde Roodt, Adolfo R.
dc.creatorFernández, Julián
dc.creatorSolano, Daniela
dc.creatorLomonte, Bruno
dc.date2020-11-24T12:49:15Z
dc.date2020-11-24T12:49:15Z
dc.date2018-06
dc.date.accessioned2023-08-29T20:07:08Z
dc.date.available2023-08-29T20:07:08Z
dc.identifier0041-0101
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1704
dc.identifierhttps://doi.org/10.1016/j.toxicon.2018.04.026
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519510
dc.descriptionFil: de Roodt, Adolfo. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.
dc.descriptionFil: Fernández, Julián. Universidad de Costa Rica. Facultad de Microbiología. Instituto Clodomiro Picado; San José, Costa Rica.
dc.descriptionFil: Solano, Daniela. Universidad de Costa Rica. Facultad de Microbiología. Instituto Clodomiro Picado; San José, Costa Rica.
dc.descriptionFil: Lomonte, Bruno. Universidad de Costa Rica. Facultad de Microbiología. Instituto Clodomiro Picado; San José, Costa Rica.
dc.descriptionBothrops cotiara is a pitviper found in Southeastern Brazil and, scarcely, in the Misiones province of Argentina. In contrast to considerable information available on the venom of the Brazilian snake population, that of Misiones has received little attention. While exploring the chromatographic venom profile of Argentinean B. cotiara, a major protein peak was found which, according to a previous study, is not present in the venom of Brazilian origin. The corresponding protein was isolated by RP-HPLC, and characterized by electrophoresis, mass spectrometry, phospholipase A2 (PLA2) assay, and myotoxic activities. Representing nearly 15% of B. cotiara venom from Misiones, this protein was identified as a Lys49 PLA2 homologue. In accordance with the characteristics of this toxin family, the protein induced myotoxicity in mice and was devoid of PLA2 activity. Since previous work reported that no PLA2 or PLA2-homologues occur in B. cotiara venom of Brazilian origin, the presence of an abundant Lys49 PLA2 homologue in the venom from Misiones highlights a striking phenotypic variation in toxin expression within two populations of a single snake species inhabiting different geographic areas. The considerable proportion of B. cotiara Lys49 PLA2 homologue myotoxin in the venom alerts that skeletal muscle necrosis might be a potentially relevant consequence of eventual envenomings by this species in Misiones.
dc.languageen
dc.relationToxicon : official journal of the International Society on Toxinology
dc.rightsnone
dc.subjectBothrops
dc.subjectFosfolipasas A2
dc.subjectVenenos de Serpiente
dc.titleA myotoxic Lys49 phospholipase A2-homologue is the major component of the venom of Bothrops cotiara from Misiones, Argentina
dc.typeArtículo


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