A comparison of different methods to assess the hemorrhagic activity of Bothrops venoms

dc.creatorde Roodt, Adolfo R.
dc.creatorDolab, Jorge A.
dc.creatorDokmetjian, Christian
dc.creatorLitwin, Silvana
dc.creatorSegré, Liliana
dc.creatorVidal, Juan C.
dc.date2020-11-20T16:44:37Z
dc.date2020-11-20T16:44:37Z
dc.date2000-06
dc.date.accessioned2023-08-29T20:07:07Z
dc.date.available2023-08-29T20:07:07Z
dc.identifier0041-0101
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1697
dc.identifierhttps://doi.org/10.1016/S0041-0101(99)00205-6
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519493
dc.descriptionFil: de Roodt, Adolfo R. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.
dc.descriptionFil: Dolab, Jorge A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.
dc.descriptionFil: Dokmetjian, Christian. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina
dc.descriptionFil: Litwin, Silvana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina.
dc.descriptionFil: Segré, Liliana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina
dc.descriptionFil: Vidal, Juan C. Museo Argentino de Ciencias Naturales Bernardino Rivadavia. Area lología; Argentina.
dc.descriptionThe hemorrhagic activity of Bothrops (B.) alternatus, B. ammodytoides, B. jararaca, B. jararacussu, B. moojeni and B. neuwiedii venoms from specimens captured in Argentina was assayed after i.d. injection to mice. The hemorrhagic haloes produced by each venom had different color intensities, although no significant differences were observed by measurement of the average diameters or the weight of the excised hemorrhagic haloes. Conversely, important differences were found by measuring the amount of hemoglobin extracted from excised hemorrhagic haloes of similar size produced by different venoms. The relationship between the amount of hemoglobin extracted and the weight of the excised hemorrhagic haloes was linear, with a slope (hemoglobin released per gram of hemorrhagic halo) characteristic for each venom, and proportional to the potency. On this basis, the activity of B. alternatus, B. ammodytoides and B. jararaca is similar, about 1.5 times higher than that of B. jararacussu and B. moojeni venoms and threefold higher than that of B. neuwiedii venom. Thus, measurement of the of hemoglobin released provides additional information in comparative studies, and may be used to assess the antihemorrhagic potency of antivenoms.
dc.languageen
dc.relationToxicon : official journal of the International Society on Toxinology
dc.rightsnone
dc.subjectVenenos de Serpiente
dc.subjectHemorragia
dc.subjectAntivenenos
dc.titleA comparison of different methods to assess the hemorrhagic activity of Bothrops venoms
dc.typeArtículo


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