Direct molecular identification of Trypanosoma cruzi discrete typing units in domestic and peridomestic Triatoma infestans and Triatoma sordida from the Argentine Chaco

dc.creatorMaffey, L
dc.creatorCardinal, Marta Victoria
dc.creatorOrdóñez-Krasnowski, P C
dc.creatorLanati, L A
dc.creatorLauricella, Marta A.
dc.creatorSchijman, A G
dc.creatorGürtler, Ricardo E.
dc.date2020-11-19T16:31:44Z
dc.date2020-11-19T16:31:44Z
dc.date2012-10
dc.date.accessioned2023-08-29T20:07:04Z
dc.date.available2023-08-29T20:07:04Z
dc.identifierhttp://sgc.anlis.gob.ar/handle/123456789/1684
dc.identifier10.1017/S0031182012000856
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8519472
dc.descriptionFil: Maffey, L. Universidad de Buenos Aires. Departamento de Ecología. Laboratorio de Eco-Epidemiología, Genética y Evolución; Argentina.
dc.descriptionFil: Cardinal, M. B. Universidad de Buenos Aires. Departamento de Ecología. Laboratorio de Eco-Epidemiología, Genética y Evolución; Argentina.
dc.descriptionFil: Ordóñez-Krasnowski, P. C. Universidad de Buenos Aires. Departamento de Ecología. Laboratorio de Eco-Epidemiología, Genética y Evolución; Argentina.
dc.descriptionFil: Lanati, L. A. Universidad de Buenos Aires. Departamento de Ecología. Laboratorio de Eco-Epidemiología, Genética y Evolución; Argentina.
dc.descriptionFil: Lauricella, M. A. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.
dc.descriptionFil: Schijman, A. G. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular. Laboratorio de Biología Molecular de la Enfermedad de Chagas; Argentina.
dc.descriptionFil: Gürtler, R. E. Universidad de Buenos Aires. Departamento de Ecología. Laboratorio de Eco-Epidemiología, Genética y Evolución; Argentina.
dc.descriptionWe assessed the distribution of Trypanosoma cruzi Discrete Typing Units (DTUs) in domestic and peridomestic Triatoma infestans and Triatoma sordida specimens collected in a well-defined rural area in Pampa del Indio, northeastern Argentina. Microscopically-positive bugs were randomly selected with a multi-level sampling design, and DTUs were identified using direct PCR strategies. TcVI predominated in 61% of 69 T. infestans and in 56% of 9 T. sordida. TcV was the secondary DTU in T. infestans (16%) and was found in 1 T. sordida specimen (11%). Three T. sordida (33%) were found infected with TcI, a DTU also identified in local Didelphis albiventris opossums. Mixed DTU infections occurred rarely (5%) and were detected both directly from the bugs' rectal ampoule and parasite cultures. The identified DTUs and bug collection sites of T. infestans were significantly associated. Bugs infected with TcV were almost exclusively captured in domiciles whereas those with TcVI were found similarly in domiciles and peridomiciles. All mixed infections occurred in domiciles. TcV-infected bugs fed more often on humans than on dogs, whereas TcVI-infected bugs showed the reverse pattern. T. sordida is a probable sylvatic vector of TcI linked to D. albiventris, and could represent a secondary vector of TcVI and TcV in the domestic/peridomestic cycle.
dc.formatpdf
dc.languageen
dc.publisherCambridge University Press
dc.relation#PLACEHOLDER_PARENT_METADATA_VALUE#
dc.relationdatasets
dc.relationParasitology
dc.rightsopen
dc.sourceParasitology 2012; 139(12):1570–1579
dc.subjectAnimales
dc.subjectAnimales Domésticos
dc.subjectArgentina
dc.subjectPerros
dc.subjectEnsayo de Inmunoadsorción Enzimática
dc.subjectGenotipo
dc.subjectHumanos
dc.subjectInsectos Vectores
dc.subjectReacción en Cadena de la Polimerasa
dc.subjectTriatoma
dc.subjectEnfermedad de Chagas
dc.titleDirect molecular identification of Trypanosoma cruzi discrete typing units in domestic and peridomestic Triatoma infestans and Triatoma sordida from the Argentine Chaco
dc.typeArtículo


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